@article{0910d932d4f34f32bd36d5337436bb7f,
title = "SARS-CoV-2 Epitopes following Infection and Vaccination Overlap Known Neutralizing Antibody Sites",
abstract = "Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites, but can be laborious. Herein, we employed peptide microarrays to map linear peptide epitopes (LPEs) recognized following SARS-CoV-2 infection and vaccination. LPEs detected by nonhuman primate (NHP) and patient IgMs after SARS-CoV-2 infection extensively overlapped, localized to functionally important virus regions, and aligned with reported neutralizing antibody binding sites. Similar LPE overlap occurred after infection and vaccination, with LPE clusters specific to each stimulus, where strong and conserved LPEs mapping to sites known or likely to inhibit spike protein function. Vaccine-specific LPEs tended to map to sites known or likely to be affected by structural changes induced by the proline substitutions in the mRNA vaccine{\textquoteright}s S protein. Mapping LPEs to regions of known functional importance in this manner may accelerate vaccine evaluation and discovery of targets for site-specific therapeutic interventions.",
author = "Li Yang and Te Liang and Pierson, {Lane M.} and Hongye Wang and Fletcher, {Jesse K.} and Shu Wang and Duran Bao and Lili Zhang and Zhen Huang and Wenshu Zheng and Xiaomei Zhang and Heewon Park and Yuwen Li and Robinson, {James E.} and Feehan, {Amy K.} and Lyon, {Christopher J.} and Jing Cao and Morici, {Lisa A.} and Chenzhong Li and Roy, {Chad J.} and Xiaobo Yu and Tony Hu",
note = "Funding Information: We gratefully acknowledge the Department of Pathology at Weill Cornell Medicine for providing clinical samples. This study was supported by the Department of Defense (grant number W8IXWH1910926) and National Institute of Allergy and Infectious Diseases contract (grant number HHSN272201700033I), National Institute of Child Health and Human Development grant (grant numbers R01HD090927 and R01HD103511) and National Center for Research Resources and the Office of Research Infrastructure Programs (grant numbers OD011104). We gratefully acknowledge the generous support of the Weatherhead Presidential Endowment Fund. Publisher Copyright: Copyright {\textcopyright} 2022 Li Yang et al.",
year = "2022",
doi = "10.34133/2022/9769803",
language = "English (US)",
volume = "2022",
journal = "Research",
issn = "2096-5168",
publisher = "American Association for the Advancement of Science",
}