Abstract
Single-cell/nucleus sequencing has been increasingly used to study specific cell populations. However, cells/nuclei often become diluted during isolation steps and are difficult to reconcentrate through centrifugation. Here, we present a protocol for sample enrichment using concanavalin A-conjugated magnetic beads. We describe steps for dissection, nuclei isolation, and fluorescence-activated cell sorting (FACS). We then detail procedures for nuclei enrichment and library preparation. This protocol enables efficient retrieval and enrichment of cells/nuclei following FACS and integrates into existing workflows of various 10× Genomics applications.
Original language | English (US) |
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Article number | 102595 |
Journal | STAR Protocols |
Volume | 4 |
Issue number | 4 |
DOIs | |
State | Published - Dec 15 2023 |
Keywords
- Cell Isolation
- Model Organisms
- Neuroscience
- Sequencing
- Single Cell
ASJC Scopus subject areas
- General Neuroscience
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology