Abstract
RSR13[2-(4[[(3,5-Dimethylanilino)carbonyl] methyl] phenoxy)-2-methyl propionic acid], a synthetic allosteric modifier of hemoglobin, increases O2 release from hemoglobin at low oxygen tension. The isolated blood-perfused rat heart was examined during potassium-arrest to determine the effects of RSR13 on the concentration of phosphocreatine (PCr) and adenosine triphosphate (ATP) by using 31P nuclear magnetic resonance (NMR) spectroscopy throughout an episode of low-flow ischemia. All hearts were perfused at constant flow during control (2.0 ml/min) and low-flow (0.2 ml/min) conditions. In normoxic hearts, RSR13 had no effect on either the 31P NMR spectrum or the rate-pressure product. In hearts subjected to 30 min of reduced flow, treatment with RSR13 improved mechanical function on reperfusion (p = 0.026 after 20 min; p = 0.032 after 25 min; and p = 0.045 after 30 min) at 2.0 ml/min with normokalemic blood perfusate. In potassium- arrested hearts, the rate of decrease of [ATP] was reduced in hearts exposed to RSR13 (p ≤ 0.05 between 10 and 35.8 min of ischemia except at 28.4 min) during low flow. These results indicate a protective effect of RSR13 on high- energy phosphates during low-flow ischemia and mechanical recovery after reperfusion.
Original language | English (US) |
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Pages (from-to) | 359-363 |
Number of pages | 5 |
Journal | Journal of Cardiovascular Pharmacology |
Volume | 31 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1998 |
Keywords
- Heart
- Hemoglobin
- P NMR
- RSR13
- Rat
ASJC Scopus subject areas
- Pharmacology
- Cardiology and Cardiovascular Medicine