Reversible Dissociation of Wheat Germ Ribosomal Subunits: Cation-Dependent Equilibriums and Thermodynamic Parameters

Joan M. Sperrazza; David W. Russell; Linda L. Spremulli

doi:10.1021/bi00547a001

Reversible Dissociation of Wheat Germ Ribosomal Subunits: Cation-Dependent Equilibriums and Thermodynamic Parameters

Joan M. Sperrazza, David W. Russell, Linda L. Spremulli

Molecular Genetics

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The influence of magnesium ion concentration upon the equilibrium between the wheat germ ribosome and its subunits has been studied by light scattering. The curves obtained for ribosome dissociation and subunit reassociation were identical and were independent of the origin and direction of the magnesium ion titration, suggesting that the wheat germ ribosomes are behaving as a homogeneous population equivalent to Escherichia coli type A ribosomes. Increasing K⁺ concentrations in the presence of Mg²⁺ favored ribosome dissociation. Polyamines favored subunit reassociation, with 0.1 mM spermine acting as effectively as 1 mM spermidine.

Original language	English (US)
Pages (from-to)	1053-1058
Number of pages	6
Journal	Biochemistry
Volume	19
Issue number	6
DOIs	https://doi.org/10.1021/bi00547a001
State	Published - Jan 1 1980

ASJC Scopus subject areas

Biochemistry

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abstract = "The influence of magnesium ion concentration upon the equilibrium between the wheat germ ribosome and its subunits has been studied by light scattering. The curves obtained for ribosome dissociation and subunit reassociation were identical and were independent of the origin and direction of the magnesium ion titration, suggesting that the wheat germ ribosomes are behaving as a homogeneous population equivalent to Escherichia coli type A ribosomes. Increasing K+ concentrations in the presence of Mg2+ favored ribosome dissociation. Polyamines favored subunit reassociation, with 0.1 mM spermine acting as effectively as 1 mM spermidine.",

author = "Sperrazza, {Joan M.} and Russell, {David W.} and Spremulli, {Linda L.}",

year = "1980",

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T1 - Reversible Dissociation of Wheat Germ Ribosomal Subunits

T2 - Cation-Dependent Equilibriums and Thermodynamic Parameters

AU - Sperrazza, Joan M.

AU - Russell, David W.

AU - Spremulli, Linda L.

PY - 1980/1/1

Y1 - 1980/1/1

N2 - The influence of magnesium ion concentration upon the equilibrium between the wheat germ ribosome and its subunits has been studied by light scattering. The curves obtained for ribosome dissociation and subunit reassociation were identical and were independent of the origin and direction of the magnesium ion titration, suggesting that the wheat germ ribosomes are behaving as a homogeneous population equivalent to Escherichia coli type A ribosomes. Increasing K+ concentrations in the presence of Mg2+ favored ribosome dissociation. Polyamines favored subunit reassociation, with 0.1 mM spermine acting as effectively as 1 mM spermidine.

AB - The influence of magnesium ion concentration upon the equilibrium between the wheat germ ribosome and its subunits has been studied by light scattering. The curves obtained for ribosome dissociation and subunit reassociation were identical and were independent of the origin and direction of the magnesium ion titration, suggesting that the wheat germ ribosomes are behaving as a homogeneous population equivalent to Escherichia coli type A ribosomes. Increasing K+ concentrations in the presence of Mg2+ favored ribosome dissociation. Polyamines favored subunit reassociation, with 0.1 mM spermine acting as effectively as 1 mM spermidine.

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JO - Biochemistry

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Reversible Dissociation of Wheat Germ Ribosomal Subunits: Cation-Dependent Equilibriums and Thermodynamic Parameters

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