@article{bb140ec4c90b43e4b9e3cd9948477987,
title = "Resolution doubling in light-sheet microscopy via oblique plane structured illumination",
abstract = "Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence microscope without requiring high laser powers or specialized fluorophores. However, the excitation of out-of-focus fluorescence can accelerate photobleaching and phototoxicity. In contrast, light-sheet fluorescence microscopy (LSFM) largely avoids exciting out-of-focus fluorescence, thereby enabling volumetric imaging with low photobleaching and intrinsic optical sectioning. Combining SIM with LSFM would enable gentle three-dimensional (3D) imaging at doubled resolution. However, multiple orientations of the illumination pattern, which are needed for isotropic resolution doubling in SIM, are challenging to implement in a light-sheet format. Here we show that multidirectional structured illumination can be implemented in oblique plane microscopy, an LSFM technique that uses a single objective for excitation and detection, in a straightforward manner. We demonstrate isotropic lateral resolution below 150 nm, combined with lower phototoxicity compared to traditional SIM systems and volumetric acquisition speed exceeding 1 Hz.",
author = "Bingying Chen and Chang, {Bo Jui} and Philippe Roudot and Felix Zhou and Etai Sapoznik and Madeleine Marlar-Pavey and Hayes, {James B.} and Brown, {Peter T.} and Zeng, {Chih Wei} and Talley Lambert and Friedman, {Jonathan R.} and Zhang, {Chun Li} and Burnette, {Dylan T.} and Shepherd, {Douglas P.} and Dean, {Kevin M.} and Fiolka, {Reto P.}",
note = "Funding Information: We thank the National Institutes of Health (grant nos. 1R01DK127589, 1R21HD105189, 5P30CA142543, 1RM1GM145399 and U54CA268072 to K.M.D.; MIRA R35GM125028 to D.T.B.; R35GM133522 to R.P.F.; R35GM137894 to J.R.F.; and 5R01NS117065 to C.-L.Z.). American Heart Association Graduate Student Fellowship to J.B.H. (grant no. AHA 836090). P.R. received funding from the Investissements d{\textquoteright}Avenir French Government program managed by the French National Research Agency (grant no. ANR-16-CONV-0001) and from Excellence Initiative of Aix-Marseille University: A*MIDEX.{\textquoteright} The SIM code development was supported by Scialog, the Research Corporation for Science Advancement, the Frederick Gardner Cottrell Foundation (grant no. 28041) and Chan Zuckerberg Initiative (grant no. 2021-236170(5022)) to D.P.S. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2022",
month = nov,
doi = "10.1038/s41592-022-01635-8",
language = "English (US)",
volume = "19",
pages = "1419--1426",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "11",
}