@article{7b972bceb1cf43bd873d5235fdeccf14,
title = "Resistin-like Molecule α Provides Vitamin-A-Dependent Antimicrobial Protection in the Skin",
abstract = "Vitamin A regulates skin immunity via unknown mechanisms. Harris et al. show that RELMα is an antimicrobial protein expressed by epidermal cells that shapes resident skin bacterial communities and limits skin infection. RELMα is induced by dietary vitamin A and mediates vitamin-A-dependent resistance to skin infection.",
keywords = "antimicrobial protein, bacteria, innate immunity, microbiome, skin, skin infection, vitamin A",
author = "Harris, {Tamia A.} and Sureka Gattu and Propheter, {Daniel C.} and Zheng Kuang and Shai Bel and Ruhn, {Kelly A.} and Chara, {Andrew L.} and Marshall Edwards and Chenlu Zhang and Jo, {Jay Hyun} and Prithvi Raj and Zouboulis, {Christos C.} and Kong, {Heidi H.} and Segre, {Julia A.} and Hooper, {Lora V.}",
note = "Funding Information: We thank C.L. Behrendt-Boyd, T. Leal, and B. Hassell for their assistance with mouse experiments; S. Muhkerjee for her assistance with protein isolation and the liposome experiments; B. Chong for human skin samples; G. Lui for the staphyloxanthin-deficient Staphylococcus aureus strain; and T. Vandergriff for review of the skin histology. This work was supported by NIH grant R01 DK070855 (L.V.H.), a Burroughs Wellcome Foundation Investigators in the pathogenesis of Infectious Diseases Award (L.V.H.), a Welch Foundation (grant I-1874 to L.V.H.), the Walter M. and Helen D. Bader Center for Research on Arthritis and Autoimmune Diseases (L.V.H.), and the Howard Hughes Medical Institute (L.V.H.). S.G. was supported by NIH grant T32 AI007520 , D.C.P. was supported by NIH grants T32 AI007520 and F32 DK100074 , Z.K. was supported by NIH grant T32 AI005284 , and S.B. was supported by a Gruss-Lipper postdoctoral fellowship. J.-H.J. was supported by a grant from the Korean Health Technology R&D Project , Ministry of Health and Welfare , Republic of Korea ( HI15C1095 ) and the Intramural Research Program of the National Cancer Institute . H.H.K. was supported by the Intramural Research Programs of the National Cancer Institute, National Institute of Arthritis and Musculoskeletal and Skin Diseases . J.A.S was supported by the Intramural Research Programs of the National Human Genome Research Institute . T.A.H. was supported by a Dermatology Foundation Career Development Award, the UT Southwestern Disease Oriented Clinical Scholars Program, a Burroughs Wellcome Fund Career Award for Medical Scientists, and a Burroughs Wellcome Fund Postdoctoral Enrichment Program Award. Funding Information: We thank C.L. Behrendt-Boyd, T. Leal, and B. Hassell for their assistance with mouse experiments; S. Muhkerjee for her assistance with protein isolation and the liposome experiments; B. Chong for human skin samples; G. Lui for the staphyloxanthin-deficient Staphylococcus aureus strain; and T. Vandergriff for review of the skin histology. This work was supported by NIH grant R01 DK070855 (L.V.H.), a Burroughs Wellcome Foundation Investigators in the pathogenesis of Infectious Diseases Award (L.V.H.), a Welch Foundation (grant I-1874 to L.V.H.), the Walter M. and Helen D. Bader Center for Research on Arthritis and Autoimmune Diseases (L.V.H.), and the Howard Hughes Medical Institute (L.V.H.). S.G. was supported by NIH grant T32 AI007520, D.C.P. was supported by NIH grants T32 AI007520 and F32 DK100074, Z.K. was supported by NIH grant T32 AI005284, and S.B. was supported by a Gruss-Lipper postdoctoral fellowship. J.-H.J. was supported by a grant from the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI15C1095) and the Intramural Research Program of the National Cancer Institute. H.H.K. was supported by the Intramural Research Programs of the National Cancer Institute, National Institute of Arthritis and Musculoskeletal and Skin Diseases. J.A.S was supported by the Intramural Research Programs of the National Human Genome Research Institute. T.A.H. was supported by a Dermatology Foundation Career Development Award, the UT Southwestern Disease Oriented Clinical Scholars Program, a Burroughs Wellcome Fund Career Award for Medical Scientists, and a Burroughs Wellcome Fund Postdoctoral Enrichment Program Award. T.A.H. designed and performed most experiments. S.G. performed experiments with SZ95 cell line in Figures 5A and 5B. C.C.Z. provided the SZ95 cells and revised the manuscript. D.C.P designed the expression constructs used in Figure 2 and performed the PI uptake assays. Z.K. performed statistical analysis of the transcriptomics data in Figures 1A, S1, and S6. S.B, A.C. K.A.R. and M.E. provided technical assistance with the mouse experiments in Figures 4 and 6. C.Z. assisted with RNA isolation and qRT-PCR analysis. J.-H.J, H.H.K, P.R. and J.A.S. performed DNA extraction and 16S rRNA analysis in Figures 3 and S5. T.A.H. and L.V.H. designed experiments, interpreted results, and wrote the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = jun,
day = "12",
doi = "10.1016/j.chom.2019.04.004",
language = "English (US)",
volume = "25",
pages = "777--788.e8",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "6",
}