TY - JOUR
T1 - Reptilian MPR 300 is also the IGF-IIR
T2 - Cloning, sequencing and functional characterization of the IGF-II binding domain
AU - Sivaramakrishna, Yadavalli
AU - Amancha, Praveen kumar
AU - Siva Kumar, Nadimpalli
N1 - Funding Information:
Research work on the MPR proteins in the laboratory of NSK is supported by a research grant from DST SR/SO/BB/60-2003. NSK thanks the Volkswagen Stiftung, Germany for a research support (I/78913). YSRK and APK thank CSIR, New Delhi, India for a research fellowship.
PY - 2009/6/1
Y1 - 2009/6/1
N2 - The mammalian cation-independent mannose 6-phosphate/insulin-like growth factor (IGF)-II receptor binds IGF-II with high affinity. Ligands transported by the MPR 300/IGF-IIR include IGF-II and mannose 6-phosphate-modified proteins. By targeting IGF-II to lysosomal degradation, it plays a key role in the maintenance of correct IGF-II levels in the circulation and in target tissues. Although, from our studies we found homologous receptor in calotes but its functional significance was not known. We present here the first report on the calotes MPR 300/IGF-IIR binds IGF-II with Kd of 12.02 nM; these findings provide new and strong evidence that MPR 300/IGF-IIR in Calotes versicolor binds IGFII with high affinity.
AB - The mammalian cation-independent mannose 6-phosphate/insulin-like growth factor (IGF)-II receptor binds IGF-II with high affinity. Ligands transported by the MPR 300/IGF-IIR include IGF-II and mannose 6-phosphate-modified proteins. By targeting IGF-II to lysosomal degradation, it plays a key role in the maintenance of correct IGF-II levels in the circulation and in target tissues. Although, from our studies we found homologous receptor in calotes but its functional significance was not known. We present here the first report on the calotes MPR 300/IGF-IIR binds IGF-II with Kd of 12.02 nM; these findings provide new and strong evidence that MPR 300/IGF-IIR in Calotes versicolor binds IGFII with high affinity.
KW - Cation-independent mannose 6-phosphate receptor
KW - Evolution
KW - IGF-II binding
KW - M6P/IGF-II receptor
KW - Reptiles (calotes)
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U2 - 10.1016/j.ijbiomac.2009.03.004
DO - 10.1016/j.ijbiomac.2009.03.004
M3 - Article
C2 - 19428478
AN - SCOPUS:67349284674
SN - 0141-8130
VL - 44
SP - 435
EP - 440
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
IS - 5
ER -