Regulation of the Rhp26ERCC6/CSB chromatin remodeler by a novel conserved leucine latch motif

Lanfeng Wang, Oliver Limbo, Jia Fei, Lu Chen, Bong Kim, Jie Luo, Jenny Chong, Ronald C. Conaway, Joan W. Conaway, Jeff A. Ranish, James T. Kadonaga, Paul Russell, Dong Wang

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


CSB/ERCC6 (Cockayne syndrome B protein/excision repair cross-complementation group 6), a member of a subfamily of SWI2/SNF2 (SWItch/sucrose nonfermentable)-related chromatin remodelers, plays crucial roles in gene expression and the maintenance of genome integrity. Here, we report the mechanism of the autoregulation of Rhp26, which is the homolog of CSB/ERCC6 in Schizosaccharomyces pombe. We identified a novel conserved protein motif, termed the "leucine latch," at the N terminus of Rhp26. The leucine latch motif mediates the autoinhibition of the ATPase and chromatin-remodeling activities of Rhp26 via its interaction with the core ATPase domain. Moreover, we found that the C terminus of the protein counteracts this autoinhibition and that both the N- and C-terminal regions of Rhp26 are needed for its proper function in DNA repair in vivo. The presence of the leucine latch motif in organisms ranging from yeast to humans suggests a conserved mechanism for the autoregulation of CSB/ERCC6 despite the otherwise highly divergent nature of the N- and C-terminal regions.

Original languageEnglish (US)
Pages (from-to)18566-18571
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number52
StatePublished - Dec 30 2014
Externally publishedYes


  • Chromatin remodeling
  • Enzyme autoregulation
  • Flanking regions
  • SNF2-like family atpase
  • SWI2/SNF2

ASJC Scopus subject areas

  • General


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