Abstract
Klotho-hypomorphic (Klothohm) mice suffer from renal salt wasting and hypovolemia despite hyperaldosteronism. The present study explored the effect of Klotho on renal Na+/K+ ATPase activity. According to immunohistochemistry and confocal microscopy Na+/K + ATPase protein abundance in isolated collecting ducts was lower in Klothohm mice than in their wild type littermates (Klotho +/+). Analysis with dual electrode voltage clamp recording showed that expression of Klotho in Xenopus oocytes increased the Na+/K + ATPase pump current. Treatment of Xenopus oocytes with Klotho protein similarly increased the pump current. In conclusion, Klotho increases the membrane abundance and activity of the Na+/K+ ATPase. Decreased Na+/K+ ATPase activity could thus contribute to the volume-depletion of klothohm mice.
Original language | English (US) |
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Pages (from-to) | 1759-1764 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 585 |
Issue number | 12 |
DOIs | |
State | Published - Jun 23 2011 |
Keywords
- Protein stability
- Pump activity
- Salt depletion
- Sodium/potassium ATPase
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology