Abstract
The β1 integrin VLA-4 (α4β1, CD49d/CD29), which is expressed on a large subpopulation of peripheral blood T lymphocytes, functions as a receptor for the endothelial adhesion protein VCAM-1 and the extracellular matrix protein fibronectin. Previous studies showed that immobilized fibronectin enhanced anti-CD3 monoclonal antibody (mAb)-induced T cell proliferation through binding to the integrins VLA-4 and VLA-5 (α5β1, CD49e/CD29). We studied the ability of the anti-CD49d mAb L25 to potentiate proliferation. T cell proliferation was induced by subthreshold concentrations of anti-CD3 mAb (mAb OKT3) coimmobilized with mAb L25 but not with coimmobilized anti-CD29 (β1) mAb. Soluble anti-CD29 mAb inhibited the proliferation induced by coimmobilized mAb OKT3 and L25 but not proliferation induced by mAb OKT3 with PMA or coimmobilized anti-CD26 mAb.
Original language | English (US) |
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Pages (from-to) | 456-462 |
Number of pages | 7 |
Journal | Journal of Leukocyte Biology |
Volume | 52 |
Issue number | 4 |
DOIs | |
State | Published - 1992 |
Keywords
- CD29
- CD49d
- VLA-4
- integrins
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology