Regulation of cardiac Na+,Ca2+ exchange and K(ATP) potassium channels by PIP2

Donald W. Hilgemann, Rebecca Ball

Research output: Contribution to journalArticlepeer-review

569 Scopus citations


Cardiac Na+,Ca2+ exchange is activated by a mechanism that requires hydrolysis of adenosine triphosphate (ATP) but is not mediated by protein kinases. In giant cardiac membrane patches, ATP acted to generate phosphatidylinositol-4,5-bisphosphate (PIP2) from phosphatidylinositol (PI). The action of ATP was abolished by a PI-specific phospholipase C (PLC) and recovered after addition of exogenous PI; it was reversed by a PIP2-specific PLC; and it was mimicked by exogenous PIP2. High concentrations of free Ca2+ (5 to 20 μM) accelerated reversal of the ATP effect, and PLC activity in myocyte membranes was activated with a similar Ca2+ dependence. Aluminum reversed the ATP effect by binding with high affinity to PIP2. ATP-inhibited potassium channels (K(ATP)) were also sensitive to PIP2, whereas Na+, K+ pumps and Na+ channels were not. Thus, PIP2 may be an important regulator of both ion transporters and channels.

Original languageEnglish (US)
Pages (from-to)956-959
Number of pages4
Issue number5277
StatePublished - Aug 16 1996

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Regulation of cardiac Na+,Ca2+ exchange and K(ATP) potassium channels by PIP2'. Together they form a unique fingerprint.

Cite this