Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells

Tracy L. Stevens, Alexis Bossie, Virginia M. Sanders, Rafael Fernandez-Botran, Robert L. Coffman, Timothy R. Mosmann, Ellen S. Vitetta

Research output: Contribution to journalArticlepeer-review

782 Scopus citations


The regulation of the subclass of immunoglobulin secreted by B cells has been studied in vitro in polyclonal systems using mitogens, such as lipopolysaccharide (LPS), to bypass the requirement for cognate interaction between antigen-specific T and B cells. In these systems, interleukin-(IL)-4 induces the secretion of IgG1, (ref. 1) and IgE (ref. 2); IL-5 enhances the secretion of IgA3,4, and interferon-γ (IFN-γ) enhances the secretion of IgG2a (ref. 5). Clones of murine TH cells can be divided into two subsets, TH1 and TH2 (ref. 6). Both subsets synthesize IL-3 and granulocyte-monocyte colony-stimulating factor (GM-CSF), but only TH1 clones produce IL-2, IFN-γ, and lymphotoxin (LT) and TH2 clones produce IL-4 and IL-5 (ref. 7). We have examined the role of clones of antigen-specific TH1 and TH2 cells in the regulation of the subclasses of IgG antibody secreted by antigen-specific B cells. Our results show that both types of TH cells induce the secretion of IgM and IgG3, whereas clones of TH1 and TH2 cells specifically induce antigen-specific B cells to secrete IgG2a and IgG1, respectively. We also demonstrate that regulation of commitment to the secretion of a particular IgG isotype occurs in two distinct stages: cognate interaction between T and B cells and interaction between T-cell-derived lymphokines and B cells.

Original languageEnglish (US)
Pages (from-to)255-258
Number of pages4
Issue number6179
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • General


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