Redox-Dependent Modulation of Aconitase Activity in Intact Mitochondria

Anne Laure Bulteau, Masao Ikeda-Saito, Luke I. Szweda

Research output: Contribution to journalArticlepeer-review

201 Scopus citations


It has previously been reported that exposure of purified mitochondrial or cytoplasmic aconitase to superoxide (O2-.) or hydrogen peroxide (H2O2) leads to release of the Fe-α from the enzyme's [4Fe-4S]2+ cluster and to inactivation. Nevertheless, little is known regarding the response of aconitase to pro-oxidants within intact mitochondria. In the present study, we provide evidence that aconitase is rapidly inactivated and subsequently reactivated when isolated cardiac mitochondria are treated with H2O2. Reactivation of the enzyme is dependent on the presence of the enzyme's substrate, citrate. EPR spectroscopic analysis indicates that enzyme inactivation precedes release of the labile Fe-α from the enzyme's [4Fe-4S]2+ cluster. In addition, as judged by isoelectric focusing gel electrophoresis, the relative level of Fe-α release and cluster disassembly does not reflect the magnitude of enzyme inactivation. These observations suggest that some form of posttranslational modification of aconitase other than release of iron is responsible for enzyme inactivation. In support of this conclusion, H 2O2 does not exert its inhibitory effects by acting directly on the enzyme, rather inactivation appears to result from interaction-(s) between aconitase and a mitochondrial membrane component responsive to H2O2. Nevertheless, prolonged exposure of mitochondria to steady-state levels of H2O2 or O 2-. results in disassembly of the [4Fe-4S]2+ cluster, carbonylation, and protein degradation. Thus, depending on the pro-oxidant species, the level and duration of the oxidative stress, and the metabolic state of the mitochondria, aconitase may undergo reversible modulation in activity or progress to [4Fe-4S]2+ cluster disassembly and proteolytic degradation.

Original languageEnglish (US)
Pages (from-to)14846-14855
Number of pages10
Issue number50
StatePublished - Dec 23 2003

ASJC Scopus subject areas

  • Biochemistry


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