@article{87300666975a4bed98ba6eee6690014d,
title = "Reconstituting Mouse Lungs with Conditionally Reprogrammed Human Bronchial Epithelial Cells",
abstract = "We developed methods for conditionally reprogramming (CR) primary human bronchial epithelial cells (HBECs) to extend their functional lifespan and permit their differentiation into both upper and lower airway lung epithelium. We also developed a bioreactor to support vascular perfusion and rhythmic breathing of decellularized mouse lungs reconstituted with CR HBECs isolated from patients with and without cystic fibrosis (CF). While conditionally reprogrammed cells only differentiate into an upper airway epithelium after 35 days at the air-liquid interface, in reconstituted lungs these cells differentiate into upper airway bronchial epithelium and lower airway alveolar structures after 12 days. Rapid scale-up and the ability to obtain clonal derivatives of primary patient-derived HBECs without the need for genetic manipulation may permit rapid reconstitution of the lung epithelium; facilitating the study of lung disease in tissue-engineered models.",
keywords = "Cystic fibrosis, Decellularized lung, Multipotent, ROCK inhibitor, Tissue engineering",
author = "Ryan Laranger and Peters-Hall, {Jennifer R.} and Melissa Coquelin and Alabi, {Busola R.} and Chen, {Christopher T.} and Wright, {Woodring E.} and Shay, {Jerry W.}",
note = "Funding Information: This work was supported by the National Cancer Institute (Lung SPORE P50CA70907 to JWS), a pilot grant from the Cystic Fibrosis Foundation (to JWS), a Cystic Fibrosis Foundation Postdoctoral Award (PETERS15FO), and NCIdesignated Comprehensive Cancer Center Support Grant (5P30 CA142543). This work was also funded by the HHMI Med into Grad Scholars Program, The TL1 Scholars Program (TL1TR001104), and a National Cancer Institute T32 training grant (CA124334) to JP-H and RL. This work was performed in laboratories constructed with support from the National Institute of Health grant C06 RR30414. Immunofluorescence microscopy was supported by NH S10 RR029731-01 to Kate Luby-Phelps Funding Information: This work was supported by the National Cancer Institute (Lung SPORE P50CA70907 to JWS), a pilot grant from the Cystic Fibrosis Foundation (to JWS), a Cystic Fibrosis Foundation Postdoctoral Award (PETERS15FO), and NCI-designated Comprehensive Cancer Center Support Grant (5P30 CA142543). This work was also funded by the HHMI Med into Grad Scholars Program, The TL1 Scholars Program (TL1TR001104), and a National Cancer Institute T32 training grant (CA124334) to JP-H and RL. This work was performed in laboratories constructed with support from the National Institute of Health grant C06 RR30414. Immunofluorescence microscopy was supported by NH S10 RR029731-01 to Kate Luby-Phelps. Publisher Copyright: {\textcopyright} Copyright 2018, Mary Ann Liebert, Inc.",
year = "2018",
month = apr,
doi = "10.1089/ten.tea.2017.0114",
language = "English (US)",
volume = "24",
pages = "559--568",
journal = "Tissue Engineering - Part A",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "7-8",
}