TY - JOUR
T1 - Recent Developments in the Enzymology of Excision Repair of DNA
AU - Friedberg, Errol C.
AU - Anderson, Corrie T M
AU - Bonura, Thomas
AU - Cone, Richard
AU - Radany, Eric H.
AU - Reynolds, Richard J.
PY - 1981/1/1
Y1 - 1981/1/1
N2 - This chapter describes the details of the enzymic mechanisms of excision repair of damaged DNA. It is the incision of UV irradiated DNA by an enzyme mechanism, involving a specific DNA glycosylase that is not universal. This argument notwithstanding, even in the two biological systems just mentioned, evidence indicates that pyrimidine dimers are excised from DNA as a part of a larger oligonucleotide, whereas in all known examples of base-excision repair, the products of excision are free bases. The chapter also describes an activity in extracts of M. luteus that recognizes 8-(2-hydroxyisopropy1) purines in DNA. Preliminary evidence indicates that this activity is a true endonuclease without associated DNA glycosylase activity. Thus, it is distinctly possible that the excision repair of cell damage by hydrolysis of N-glycosyl bonds involves a number of specific forms of base damage, in which the degree of structural distortion of DNA secondary structure is limited. Very bulky DNA adducts such as those produced by the interaction of DNA with polycyclic aromatic hydrocarbons, acetamidofluorene, etc., may generate relatively nonspecific but significant distortion of the DNA helix that is recognized by a limited number of “general” endonucleases.
AB - This chapter describes the details of the enzymic mechanisms of excision repair of damaged DNA. It is the incision of UV irradiated DNA by an enzyme mechanism, involving a specific DNA glycosylase that is not universal. This argument notwithstanding, even in the two biological systems just mentioned, evidence indicates that pyrimidine dimers are excised from DNA as a part of a larger oligonucleotide, whereas in all known examples of base-excision repair, the products of excision are free bases. The chapter also describes an activity in extracts of M. luteus that recognizes 8-(2-hydroxyisopropy1) purines in DNA. Preliminary evidence indicates that this activity is a true endonuclease without associated DNA glycosylase activity. Thus, it is distinctly possible that the excision repair of cell damage by hydrolysis of N-glycosyl bonds involves a number of specific forms of base damage, in which the degree of structural distortion of DNA secondary structure is limited. Very bulky DNA adducts such as those produced by the interaction of DNA with polycyclic aromatic hydrocarbons, acetamidofluorene, etc., may generate relatively nonspecific but significant distortion of the DNA helix that is recognized by a limited number of “general” endonucleases.
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U2 - 10.1016/S0079-6603(08)60405-5
DO - 10.1016/S0079-6603(08)60405-5
M3 - Article
C2 - 6269148
AN - SCOPUS:0019344471
SN - 0079-6603
VL - 26
SP - 197
EP - 215
JO - Progress in Nucleic Acid Research and Molecular Biology
JF - Progress in Nucleic Acid Research and Molecular Biology
IS - C
ER -