Abstract
The inhibitor of γ-glutamyl transpeptidase (γ-GT) acivicin modulates cellular responses including growth, myeloid maturation and apoptosis. Whether these effects result from the inhibition of γ-GT enzyme activity remains unclear. We compared the cellular effects of acivicin against a more potent and specific inhibitor of γ-GT (L-2-amino-4-boronobutanoic acid (L-ABBA)) in γ-GT-negative (B lymphoblastoid Ramos) and γ-GT-positive (myelomonocytic HL-60, γ-GT-transfected Ramos) cell lines. Under non-oxidative stress conditions, acivicin-induced cell growth arrest, apoptosis and macrophage maturation occurred independent of γ-GT while L-ABBA did not influence any of these processes. Acivicin triggered tyrosine phosphorylation and increased nuclear factor κB activity. Further insight into the role of γ-GT in cellular processes is needed.
Original language | English (US) |
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Pages (from-to) | 226-230 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 508 |
Issue number | 2 |
DOIs | |
State | Published - Nov 16 2001 |
Keywords
- Acivicin
- Apoptosis
- Differentiation
- Ectoprotease
- Proliferation
- Signaling
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology