@article{0993dee35a3c4ad29d5bcbedbbf1c591,
title = "Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay",
abstract = "Peptide mobility shift assays provide a sensitive measure of kinase enzymatic activity and can be used to evaluate kinase inhibitors. Herein, we describe a protocol adapted for rapid assessment of doublecortin-like kinase inhibitors. Advantages include rapid iterations of therapeutic compound assessment and the ability to characterize kinase mutations, such as drug-resistant mutants for biological rescue experiments, on kinase activity. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).",
keywords = "cancer, high-throughput screening, molecular/chemical probes, protein biochemistry",
author = "Yan Liu and Westover, {Kenneth D.}",
note = "Funding Information: K.D.W. has received consulting fees from Sanofi Oncology and is a member of the SAB for Vibliome Therapeutics. K.D.W. has received research funding from Revolution Medicines. K.D.W. declares that none of these relationships are directly or indirectly related to the content of this manuscript. Funding Information: This work was supported by grants from the American Cancer Society award 132205-RSG-18-039-01-DMC (K.D.W.), Cancer Prevention and Research Institute of Texas RP170373 (K.D.W.), and Welch Foundation Grant I1829 (K.D.W.). K.D.W. designed this study, and Y.L. performed the experiments and data analysis. K.D.W. has received consulting fees from Sanofi Oncology and is a member of the SAB for Vibliome Therapeutics. K.D.W. has received research funding from Revolution Medicines. K.D.W. declares that none of these relationships are directly or indirectly related to the content of this manuscript. Funding Information: This work was supported by grants from the American Cancer Society award 132205-RSG-18-039-01-DMC (K.D.W.), Cancer Prevention and Research Institute of Texas RP170373 (K.D.W.), and Welch Foundation Grant I1829 (K.D.W.). Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100587",
language = "English (US)",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}