Abstract
Telomeres terminate in 3′ overhangs that function in end protection and the formation of t-loops. Determining the steps and factors involved in overhang processing is compromised by the inability to easily and accurately determine overhang size in the presence of many kilobases of double-stranded telomeric DNA. We here describe the use of a double-strand specific nuclease (DSN) that entirely digests double-stranded DNA including telomeres, leaving the overhangs intact so that they can be measured.
Original language | English (US) |
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Article number | e14 |
Journal | Nucleic acids research |
Volume | 36 |
Issue number | 3 |
DOIs | |
State | Published - Feb 2008 |
ASJC Scopus subject areas
- Genetics