Quantitation of Glutathione and Oxidized Glutathione Ratios from Biological Matrices Using LC-MS/MS

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Oxidation of glutathione (GSH) to its disulfide dimer (GSSG) is the major mechanism by which cells balance reactive oxygen species (ROS) and mitigate oxidative stress. Thus, measuring the ratio of GSH/GSSG is an ideal way to assess oxidative stress within a cell. Quantitative mass spectrometry offers an ideal method to measure the GSH/GSSG ratio and can be applied to a variety of biological matrices and disease models. The following chapter details the design, optimization, and execution of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay to measure the GSH/GSSG ratio.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages133-148
Number of pages16
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2675
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Glutathione
  • Liquid chromatography
  • Mass spectrometry
  • Oxidative stress
  • Oxidized glutathione
  • Quantitation
  • Reactive oxygen species

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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