Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes

Yong Cai, Jingji Jin, Aaron J. Gottschalk, Tingting Yao, Joan W. Conaway, Ronald C. Conaway

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Chromatin modifying and remodeling enzymes play critical roles in many aspects of chromosome biology including transcription, replication, recombination, and repair. Our laboratory recently identified and characterized two multisubunit human chromatin remodeling enzymes designated the INO80 and SRCAP complexes. Mechanistic studies revealed that the human INO80 complex catalyzes nucleosome sliding and the SRCAP complex catalyzes ATP-dependent exchange of histone H2A/H2B dimers containing the histone variant H2A.Z into nucleosomes. Here we describe methods for purification and assay of the INO80 and SRCAP chromatin remodeling complexes.

Original languageEnglish (US)
Pages (from-to)312-317
Number of pages6
Issue number4
StatePublished - Dec 2006
Externally publishedYes


  • Chromatin
  • Chromatin remodeler
  • Histone
  • Histone exchange
  • Nucleosome

ASJC Scopus subject areas

  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology


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