@article{e8ec699a8ee44a30813594cb85e4f344,
title = "Protocol for sleep analysis in the brain of genetically modified adult mice",
abstract = "Elucidating the molecular pathways that regulate animal behavior such as sleep is essential for understanding how the brain works. However, to examine how a certain functional domain of protein is involved in animal behavior is challenging. Here, we present a protocol for inducing endogenous protein that lacks a specific functional domain using Cre-mediated allele modification in neurons followed by electroencephalogram/electromyogram (EEG/EMG) recording to study the role of kinases in sleep. This strategy is applicable to other gene targets or behaviors. For complete details on the use and execution of this protocol, please refer to Iwasaki et al. (2021).",
keywords = "Behavior, Model Organisms, Molecular Biology, Neuroscience",
author = "Kanako Iwasaki and Noriko Hotta-Hirashima and Hiromasa Funato and Masashi Yanagisawa",
note = "Funding Information: This work was supported by Ministry of Education, Culture, Sports, Science and Technology (World Premier International Research Center Initiative to M.Y.) and Japan Society for the Promotion of Science KAKENHI (17H06095 to M.Y. and H.F.; 16K15187, 17H04023, 17H05583, and 20H00567 to H.F.; 20J12137 to K.I.). We thank Tomoyuki Fujiyama, Aya Ikkyu, Chika Miyoshi and all Yanagisawa/Funato laboratory members and IIIS members for the kind support, technical assistance, and discussion. Methodology, K.I. and N.H.-H.; investigation, K.I.; conceptualization, M.Y. and H.F.; writing, K.I. H.F. and M.Y. The authors declare no competing interests. Funding Information: This work was supported by Ministry of Education, Culture, Sports, Science and Technology (World Premier International Research Center Initiative to M.Y.) and Japan Society for the Promotion of Science KAKENHI ( 17H06095 to M.Y. and H.F.; 16K15187 , 17H04023 , 17H05583 , and 20H00567 to H.F.; 20J12137 to K.I.). We thank Tomoyuki Fujiyama, Aya Ikkyu, Chika Miyoshi and all Yanagisawa/Funato laboratory members and IIIS members for the kind support, technical assistance, and discussion. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = dec,
day = "17",
doi = "10.1016/j.xpro.2021.100982",
language = "English (US)",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",
}