Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use

Prerana Ramadurgum; John D. Hulleman

doi:10.1016/j.xpro.2020.100069

Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use

Prerana Ramadurgum, John D. Hulleman

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The use of destabilizing domains (DDs) to conditionally control the abundance of a protein of interest (POI) through a small-molecule stabilizer has gained increasing traction both in vitro and in vivo. Yet there are specific considerations for the development and accurate control of user-defined POIs via DDs, as well as the identification of novel (and potentially synergistic) small-molecule stabilizers. Here, we describe a platform for achieving these goals. For complete details on the use and execution of this protocol, please refer to Ramadurgum et al. (2020).

Original language	English (US)
Article number	100069
Journal	STAR Protocols
Volume	1
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2020.100069
State	Published - Sep 18 2020

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Immunology and Microbiology
General Medicine

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10.1016/j.xpro.2020.100069

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title = "Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use",

abstract = "The use of destabilizing domains (DDs) to conditionally control the abundance of a protein of interest (POI) through a small-molecule stabilizer has gained increasing traction both in vitro and in vivo. Yet there are specific considerations for the development and accurate control of user-defined POIs via DDs, as well as the identification of novel (and potentially synergistic) small-molecule stabilizers. Here, we describe a platform for achieving these goals. For complete details on the use and execution of this protocol, please refer to Ramadurgum et al. (2020).",

author = "Prerana Ramadurgum and Hulleman, {John D.}",

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Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use

Abstract

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