Proteomic profiles of mouse neuro N2a cells infected with variant virulence of rabies viruses

Xiaohu Wang, Shoufeng Zhang, Chenglong Sun, Zi Guo Yuan, Xianfu Wu, Dongxia Wang, Zhuang Ding, Rongliang Hu

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


We characterized the proteomes of murine N2a cells following infection with three rabies virus (RV) strains, characterized by distinct virulence phenotypes (i.e., virulent BD06, fixed CVS-11, and attenuated SRV9 strains), and identified 35 changes to protein expression using twodimensional gel electrophoresis in whole-cell lysates. The annotated functions of these proteins are involved in various cytoskeletal, signal transduction, stress response, and metabolic processes. Specifically, a-enolase, prx-4, vimentin, cytokine-induced apoptosis inhibitor 1 (CIAPIN1) and prx-6 were significantly up-regulated, whereas Trx like-1 and galectin-1 were down-regulated following infection of N2a cells with all three rabies virus strains. However, comparing expressions of all 35 proteins affected between BD06-, CVS-11-, and SRV9-infected cells, specific changes in expression were also observed. The up-regulation of vimentin, CIAPIN1, prx-4, and 14-3-3 θ/δ, and downregulation of NDPK-B and HSP-1 with CVS and SRV9 infection were ≥2 times greater than with BD06. Meanwhile, Zfp12 protein, splicing factor, and arginine/serine-rich 1 were unaltered in the cells infected with BD06 and CVS- 11, but were up-regulated in the group infected with SRV9. The proteomic alterations described here may suggest that these changes to protein expression correlate with the rabies virus' adaptability and virulence in N2a cells, and hence provides new clues as to the response of N2a host cells to rabies virus infections, and may also aid in uncovering new pathways in these cells that are involved in rabies infections. Further characterization of the functions of the affected proteins may contribute to our understanding of the mechanisms of RV infection and pathogenesis.

Original languageEnglish (US)
Pages (from-to)366-373
Number of pages8
JournalJournal of Microbiology and Biotechnology
Issue number4
StatePublished - Apr 2011
Externally publishedYes


  • Differential virulence
  • N2a cell proteome
  • Proteomics
  • Rabies virus
  • Viral infection

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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