Proinflammatory fecal mRNA and childhood bacterial enteric infections

William E. Bennett; Rosbel González-Rivera; Bao N. Puente; Nurmohammad Shaikh; Harold J. Stevens; Jody C. Mooney; Eileen J. Klein; Donna M. Denno; Andrew Draghi; Francisco A. Sylvester; Phillip I. Tarr

doi:10.4161/gmic.1.4.13004

Proinflammatory fecal mRNA and childhood bacterial enteric infections

William E. Bennett, Rosbel González-Rivera, Bao N. Puente, Nurmohammad Shaikh, Harold J. Stevens, Jody C. Mooney, Eileen J. Klein, Donna M. Denno, Andrew Draghi, Francisco A. Sylvester, Phillip I. Tarr

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Introduction: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. Results: Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. Discussion: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA. Methods: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.

Original language	English (US)
Pages (from-to)	209-212
Number of pages	4
Journal	Gut Microbes
Volume	1
Issue number	4
DOIs	https://doi.org/10.4161/gmic.1.4.13004
State	Published - 2010

Keywords

Campylobacter
E. coli O157:H7
Enzyme immunoassay
Infectious colitis
Salmonella
Shigella
Stool
mRNA

ASJC Scopus subject areas

Microbiology
Gastroenterology
Microbiology (medical)
Infectious Diseases

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10.4161/gmic.1.4.13004

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@article{fe6af154aeb348e4913b96a52d0bfb16,

title = "Proinflammatory fecal mRNA and childhood bacterial enteric infections",

abstract = "Introduction: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. Results: Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. Discussion: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA. Methods: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.",

keywords = "Campylobacter, E. coli O157:H7, Enzyme immunoassay, Infectious colitis, Salmonella, Shigella, Stool, mRNA",

author = "Bennett, {William E.} and Rosbel Gonz{\'a}lez-Rivera and Puente, {Bao N.} and Nurmohammad Shaikh and Stevens, {Harold J.} and Mooney, {Jody C.} and Klein, {Eileen J.} and Denno, {Donna M.} and Andrew Draghi and Sylvester, {Francisco A.} and Tarr, {Phillip I.}",

note = "Funding Information: This research has been supported by NIH grants NIDDK T32 DK077653-17, UH2 AIO83265, and the Doris Duke Clinical Research Fellowship Program. Specimens were obtained using resources from grants NIH DK 52081 and USDA 2002-35212-14257.",

year = "2010",

doi = "10.4161/gmic.1.4.13004",

language = "English (US)",

volume = "1",

pages = "209--212",

journal = "Gut Microbes",

issn = "1949-0976",

publisher = "Landes Bioscience",

number = "4",

}

TY - JOUR

T1 - Proinflammatory fecal mRNA and childhood bacterial enteric infections

AU - Bennett, William E.

AU - González-Rivera, Rosbel

AU - Puente, Bao N.

AU - Shaikh, Nurmohammad

AU - Stevens, Harold J.

AU - Mooney, Jody C.

AU - Klein, Eileen J.

AU - Denno, Donna M.

AU - Draghi, Andrew

AU - Sylvester, Francisco A.

AU - Tarr, Phillip I.

N1 - Funding Information: This research has been supported by NIH grants NIDDK T32 DK077653-17, UH2 AIO83265, and the Doris Duke Clinical Research Fellowship Program. Specimens were obtained using resources from grants NIH DK 52081 and USDA 2002-35212-14257.

PY - 2010

Y1 - 2010

N2 - Introduction: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. Results: Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. Discussion: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA. Methods: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.

AB - Introduction: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. Results: Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. Discussion: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA. Methods: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.

KW - Campylobacter

KW - E. coli O157:H7

KW - Enzyme immunoassay

KW - Infectious colitis

KW - Salmonella

KW - Shigella

KW - Stool

KW - mRNA

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Proinflammatory fecal mRNA and childhood bacterial enteric infections

Abstract

Keywords

ASJC Scopus subject areas

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