TY - JOUR
T1 - Primate gastrulation and early organogenesis at single-cell resolution
AU - Zhai, Jinglei
AU - Guo, Jing
AU - Wan, Haifeng
AU - Qi, Luqing
AU - Liu, Lizhong
AU - Xiao, Zhenyu
AU - Yan, Long
AU - Schmitz, Daniel A.
AU - Xu, Yanhong
AU - Yu, Dainan
AU - Wu, Xulun
AU - Zhao, Wentao
AU - Yu, Kunyuan
AU - Jiang, Xiangxiang
AU - Guo, Fan
AU - Wu, Jun
AU - Wang, Hongmei
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12/22
Y1 - 2022/12/22
N2 - Our understanding of human early development is severely hampered by limited access to embryonic tissues. Due to their close evolutionary relationship with humans, nonhuman primates are often used as surrogates to understand human development but currently suffer from a lack of in vivo datasets, especially from gastrulation to early organogenesis during which the major embryonic cell types are dynamically specified. To fill this gap, we collected six Carnegie stage 8–11 cynomolgus monkey (Macaca fascicularis) embryos and performed in-depth transcriptomic analyses of 56,636 single cells. Our analyses show transcriptomic features of major perigastrulation cell types, which help shed light on morphogenetic events including primitive streak development, somitogenesis, gut tube formation, neural tube patterning and neural crest differentiation in primates. In addition, comparative analyses with mouse embryos and human embryoids uncovered conserved and divergent features of perigastrulation development across species—for example, species-specific dependency on Hippo signalling during presomitic mesoderm differentiation—and provide an initial assessment of relevant stem cell models of human early organogenesis. This comprehensive single-cell transcriptome atlas not only fills the knowledge gap in the nonhuman primate research field but also serves as an invaluable resource for understanding human embryogenesis and developmental disorders.
AB - Our understanding of human early development is severely hampered by limited access to embryonic tissues. Due to their close evolutionary relationship with humans, nonhuman primates are often used as surrogates to understand human development but currently suffer from a lack of in vivo datasets, especially from gastrulation to early organogenesis during which the major embryonic cell types are dynamically specified. To fill this gap, we collected six Carnegie stage 8–11 cynomolgus monkey (Macaca fascicularis) embryos and performed in-depth transcriptomic analyses of 56,636 single cells. Our analyses show transcriptomic features of major perigastrulation cell types, which help shed light on morphogenetic events including primitive streak development, somitogenesis, gut tube formation, neural tube patterning and neural crest differentiation in primates. In addition, comparative analyses with mouse embryos and human embryoids uncovered conserved and divergent features of perigastrulation development across species—for example, species-specific dependency on Hippo signalling during presomitic mesoderm differentiation—and provide an initial assessment of relevant stem cell models of human early organogenesis. This comprehensive single-cell transcriptome atlas not only fills the knowledge gap in the nonhuman primate research field but also serves as an invaluable resource for understanding human embryogenesis and developmental disorders.
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U2 - 10.1038/s41586-022-05526-y
DO - 10.1038/s41586-022-05526-y
M3 - Article
C2 - 36517595
AN - SCOPUS:85143845809
SN - 0028-0836
VL - 612
SP - 732
EP - 738
JO - Nature
JF - Nature
IS - 7941
ER -