TY - JOUR
T1 - Prevention of alcohol-induced learning deficits in fetal alcohol syndrome mediated through NMDA and GABA receptors
AU - Toso, Laura
AU - Poggi, Sarah H.
AU - Roberson, Robin
AU - Woodard, Jade
AU - Park, Jane
AU - Abebe, Daniel
AU - Spong, Catherine Y.
N1 - Funding Information:
This work was supported by the Division of Intramural Research of National Institute of Child and Human Development.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/3
Y1 - 2006/3
N2 - Objective: Vasoactive intestinal peptide (VIP)-related peptides prevented the learning deficit in the offspring in a model for fetal alcohol syndrome. We evaluated whether the mechanism of the peptide protection included NR2B, NR2A, and GABAAα5. Study design: Timed, pregnant C57BL6/J mice were injected on gestational day 8 with alcohol (0.03 mL/kg), placebo, or alcohol plus peptides. Embryos were harvested after 6 hours, 24 hours, and on gestational day 18. Some of the litters were allowed to deliver, and the adult brains harvested after the offspring were tested for learning. Calibrator-normalized relative real-time polymerase chain reaction (PCR) was performed using primers for NR2B, NR2A, and GABAAα5 with GAPDH standardization. Statistic: analysis of variance (ANOVA) and Fisher PLSD, P < .05 was considered significant. Results: In the embryo, the peptides prevented NR2B rise (P < .001) at 6 hours, NR2B down-regulation (P = .002), and GABAAα5 decrease (P < .01) on gestational day 18. In the adult, the peptides prevented NR2B down-regulation (P = .01) and NR2A up-regulation (P < .001). Conclusion: VIP-related peptides prevented alcohol-induced changes in NR2B, NR2A, and GABAAα 5. This may explain, at least in part, the peptides' prevention of alcohol-induced learning deficits.
AB - Objective: Vasoactive intestinal peptide (VIP)-related peptides prevented the learning deficit in the offspring in a model for fetal alcohol syndrome. We evaluated whether the mechanism of the peptide protection included NR2B, NR2A, and GABAAα5. Study design: Timed, pregnant C57BL6/J mice were injected on gestational day 8 with alcohol (0.03 mL/kg), placebo, or alcohol plus peptides. Embryos were harvested after 6 hours, 24 hours, and on gestational day 18. Some of the litters were allowed to deliver, and the adult brains harvested after the offspring were tested for learning. Calibrator-normalized relative real-time polymerase chain reaction (PCR) was performed using primers for NR2B, NR2A, and GABAAα5 with GAPDH standardization. Statistic: analysis of variance (ANOVA) and Fisher PLSD, P < .05 was considered significant. Results: In the embryo, the peptides prevented NR2B rise (P < .001) at 6 hours, NR2B down-regulation (P = .002), and GABAAα5 decrease (P < .01) on gestational day 18. In the adult, the peptides prevented NR2B down-regulation (P = .01) and NR2A up-regulation (P < .001). Conclusion: VIP-related peptides prevented alcohol-induced changes in NR2B, NR2A, and GABAAα 5. This may explain, at least in part, the peptides' prevention of alcohol-induced learning deficits.
KW - Alcohol
KW - Fetal alcohol syndrome
KW - GABA
KW - Learning
KW - NMDA
KW - Neurotrophic factors
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U2 - 10.1016/j.ajog.2006.01.003
DO - 10.1016/j.ajog.2006.01.003
M3 - Article
C2 - 16522397
AN - SCOPUS:33644675566
SN - 0002-9378
VL - 194
SP - 681
EP - 686
JO - American journal of obstetrics and gynecology
JF - American journal of obstetrics and gynecology
IS - 3
ER -