Abstract
Platelet-derived growth factor receptor β (PDGFRβ) in fibroblasts is concentrated in caveolae where it controls the tyrosine phosphorylation of multiple proteins. Caveolae are enriched in cholesterol and sphingolipids, but the role of these lipids in PDGFR signal transduction is unknown. We report that introduction of cholest-4-en-3-one into caveolae membranes uncouples PDGFR autophosphorylation from tyrosine phosphorylation of neighboring proteins. Cholest-4-en-3-one appears to interfere with the normal interaction between PDGFR and its partners. The results suggest that tightly packed caveolae lipids form a membrane platform that functions as a lipid scaffold for organizing the molecular interactions of multiple signaling pathways.
Original language | English (US) |
---|---|
Pages (from-to) | 31648-31654 |
Number of pages | 7 |
Journal | Journal of Biological Chemistry |
Volume | 275 |
Issue number | 41 |
DOIs | |
State | Published - Oct 13 2000 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology