Preparation of the mineral phase of bone using ethylenediamine extraction

H. Catherine W Skinner; Ellis S. Kempner; Charles Y C Pak

doi:10.1007/BF02012557

Preparation of the mineral phase of bone using ethylenediamine extraction

H. Catherine W Skinner, Ellis S. Kempner, Charles Y C Pak

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A detailed, efficient method of extracting mineral matter from bone tissue using a Soxhlet apparatus and anhydrous ethylenediamine is presented. The ethylenediamine solubilizes the organic portion, fat and protein, of the tissue with minor, if any, chemical or physical alteration of the mineral phase. After ethylenediamine treatment the sample is washed with cold water to eliminate the solvent. X-ray diffraction, density and chemical analyses and exchange studies have been used to monitor changes within the mineral matter during the extraction and washing procedures.

Original language	English (US)
Pages (from-to)	257-268
Number of pages	12
Journal	Calcified Tissue Research
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/BF02012557
State	Published - Dec 1 1972

Keywords

Anorganic tissue
Bone
Ethylenediamine
Hydroxyapatite

ASJC Scopus subject areas

Endocrinology

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10.1007/BF02012557

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abstract = "A detailed, efficient method of extracting mineral matter from bone tissue using a Soxhlet apparatus and anhydrous ethylenediamine is presented. The ethylenediamine solubilizes the organic portion, fat and protein, of the tissue with minor, if any, chemical or physical alteration of the mineral phase. After ethylenediamine treatment the sample is washed with cold water to eliminate the solvent. X-ray diffraction, density and chemical analyses and exchange studies have been used to monitor changes within the mineral matter during the extraction and washing procedures.",

keywords = "Anorganic tissue, Bone, Ethylenediamine, Hydroxyapatite",

author = "Skinner, {H. Catherine W} and Kempner, {Ellis S.} and Pak, {Charles Y C}",

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AU - Skinner, H. Catherine W

AU - Kempner, Ellis S.

AU - Pak, Charles Y C

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Y1 - 1972/12/1

N2 - A detailed, efficient method of extracting mineral matter from bone tissue using a Soxhlet apparatus and anhydrous ethylenediamine is presented. The ethylenediamine solubilizes the organic portion, fat and protein, of the tissue with minor, if any, chemical or physical alteration of the mineral phase. After ethylenediamine treatment the sample is washed with cold water to eliminate the solvent. X-ray diffraction, density and chemical analyses and exchange studies have been used to monitor changes within the mineral matter during the extraction and washing procedures.

AB - A detailed, efficient method of extracting mineral matter from bone tissue using a Soxhlet apparatus and anhydrous ethylenediamine is presented. The ethylenediamine solubilizes the organic portion, fat and protein, of the tissue with minor, if any, chemical or physical alteration of the mineral phase. After ethylenediamine treatment the sample is washed with cold water to eliminate the solvent. X-ray diffraction, density and chemical analyses and exchange studies have been used to monitor changes within the mineral matter during the extraction and washing procedures.

KW - Anorganic tissue

KW - Bone

KW - Ethylenediamine

KW - Hydroxyapatite

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Preparation of the mineral phase of bone using ethylenediamine extraction

Abstract

Keywords

ASJC Scopus subject areas

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