Preparation of the mineral phase of bone using ethylenediamine extraction

H. Catherine W Skinner, Ellis S. Kempner, Charles Y C Pak

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


A detailed, efficient method of extracting mineral matter from bone tissue using a Soxhlet apparatus and anhydrous ethylenediamine is presented. The ethylenediamine solubilizes the organic portion, fat and protein, of the tissue with minor, if any, chemical or physical alteration of the mineral phase. After ethylenediamine treatment the sample is washed with cold water to eliminate the solvent. X-ray diffraction, density and chemical analyses and exchange studies have been used to monitor changes within the mineral matter during the extraction and washing procedures.

Original languageEnglish (US)
Pages (from-to)257-268
Number of pages12
JournalCalcified Tissue Research
Issue number1
StatePublished - Dec 1 1972


  • Anorganic tissue
  • Bone
  • Ethylenediamine
  • Hydroxyapatite

ASJC Scopus subject areas

  • Endocrinology


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