Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex

Kim S. Lau; Jun Lee; Charles W. Fisher; Rody P. Cox; David T. Chuang

doi:10.1016/0014-5793(91)80155-V

Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex

Kim S. Lau, Jun Lee, Charles W. Fisher, Rody P. Cox, David T. Chuang

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.

Original language	English (US)
Pages (from-to)	229-232
Number of pages	4
Journal	FEBS Letters
Volume	279
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)80155-V
State	Published - Feb 25 1991

Keywords

Alternative splicing
E2 gene
Genomic clone
cDNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)80155-V

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title = "Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex",

abstract = "We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.",

keywords = "Alternative splicing, E2 gene, Genomic clone, cDNA",

author = "Lau, {Kim S.} and Jun Lee and Fisher, {Charles W.} and Cox, {Rody P.} and Chuang, {David T.}",

note = "Funding Information: ,,Ickt~ow/edBements,, Tiffs work was supported by Grants DK26758 and DK37373 from th~ National Institutes of Health, Grant.in.Aid 909-093, American H~art Association. Texas Affiliate and Grant 1-1149 from the Marcl~ of Dimes Birth Defects Foundation. J.L, was a recipient of a Chillon Foundation Fellowship, We thank Dr R. Padgett for helpful discussions",

year = "1991",

month = feb,

day = "25",

doi = "10.1016/0014-5793(91)80155-V",

language = "English (US)",

volume = "279",

pages = "229--232",

journal = "FEBS Letters",

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T1 - Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex

AU - Lau, Kim S.

AU - Lee, Jun

AU - Fisher, Charles W.

AU - Cox, Rody P.

AU - Chuang, David T.

N1 - Funding Information: ,,Ickt~ow/edBements,, Tiffs work was supported by Grants DK26758 and DK37373 from th~ National Institutes of Health, Grant.in.Aid 909-093, American H~art Association. Texas Affiliate and Grant 1-1149 from the Marcl~ of Dimes Birth Defects Foundation. J.L, was a recipient of a Chillon Foundation Fellowship, We thank Dr R. Padgett for helpful discussions

PY - 1991/2/25

Y1 - 1991/2/25

N2 - We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.

AB - We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.

KW - Alternative splicing

KW - E2 gene

KW - Genomic clone

KW - cDNA

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Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex

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