Precision modulation of dysbiotic adult microbiomes with a human-milk-derived synbiotic reshapes gut microbial composition and metabolites

Julie E. Button, Casey M. Cosetta, Abigail L. Reens, Sarah L. Brooker, Aislinn D. Rowan-Nash, Richard C. Lavin, Russell Saur, Shuning Zheng, Chloe A. Autran, Martin L. Lee, Adam K. Sun, Amin M. Alousi, Christine B. Peterson, Andrew Y. Koh, David J. Rechtman, Robert R. Jenq, Gregory J. McKenzie

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Manipulation of the gut microbiome using live biotherapeutic products shows promise for clinical applications but remains challenging to achieve. Here, we induced dysbiosis in 56 healthy volunteers using antibiotics to test a synbiotic comprising the infant gut microbe, Bifidobacterium longum subspecies infantis (B. infantis), and human milk oligosaccharides (HMOs). B. infantis engrafted in 76% of subjects in an HMO-dependent manner, reaching a relative abundance of up to 81%. Changes in microbiome composition and gut metabolites reflect altered recovery of engrafted subjects compared with controls. Engraftment associates with increases in lactate-consuming Veillonella, faster acetate recovery, and changes in indolelactate and p-cresol sulfate, metabolites that impact host inflammatory status. Furthermore, Veillonella co-cultured in vitro and in vivo with B. infantis and HMO converts lactate produced by B. infantis to propionate, an important mediator of host physiology. These results suggest that the synbiotic reproducibly and predictably modulates recovery of a dysbiotic microbiome.

Original languageEnglish (US)
Pages (from-to)1523-1538.e10
JournalCell Host and Microbe
Volume31
Issue number9
DOIs
StatePublished - Sep 13 2023

Keywords

  • B. infantis
  • Bifidobacterium
  • HMO
  • LBP
  • Veillonella
  • gut engraftment
  • gut microbiome
  • gut microbiota
  • human milk oligosaccharides
  • live biotherapeutic product
  • microbiome modulation
  • propionate

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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