Precisely ordered phosphorylation reactions in the p38 mitogen-activated protein (MAP) kinase cascade

John M. Humphreys; Alexander T. Piala; Radha Akella; Haixia He; Elizabeth J. Goldsmith

doi:10.1074/jbc.M113.462101

Precisely ordered phosphorylation reactions in the p38 mitogen-activated protein (MAP) kinase cascade

John M. Humphreys, Alexander T. Piala, Radha Akella, Haixia He, Elizabeth J. Goldsmith

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Background: MAPK cascades are signaling modules that function as switch generators. Results: In vitro phosphorylation in the p38 MAPK cascade tracked by LC-MS/MS revealed specific phosphorylation intermediates at each level. Conclusion: The p38 MAPK cascade reactions occur through intermediates MEK6/ST* and p38α/TY*. Significance: The precise order of reactions may contribute to the diverse kinetic outputs of the cascades, including those with large Hill coefficients.

Original language	English (US)
Pages (from-to)	23322-23330
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	288
Issue number	32
DOIs	https://doi.org/10.1074/jbc.M113.462101
State	Published - Aug 9 2013

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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abstract = "Background: MAPK cascades are signaling modules that function as switch generators. Results: In vitro phosphorylation in the p38 MAPK cascade tracked by LC-MS/MS revealed specific phosphorylation intermediates at each level. Conclusion: The p38 MAPK cascade reactions occur through intermediates MEK6/ST* and p38α/TY*. Significance: The precise order of reactions may contribute to the diverse kinetic outputs of the cascades, including those with large Hill coefficients.",

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AU - Humphreys, John M.

AU - Piala, Alexander T.

AU - Akella, Radha

AU - He, Haixia

AU - Goldsmith, Elizabeth J.

PY - 2013/8/9

Y1 - 2013/8/9

N2 - Background: MAPK cascades are signaling modules that function as switch generators. Results: In vitro phosphorylation in the p38 MAPK cascade tracked by LC-MS/MS revealed specific phosphorylation intermediates at each level. Conclusion: The p38 MAPK cascade reactions occur through intermediates MEK6/ST* and p38α/TY*. Significance: The precise order of reactions may contribute to the diverse kinetic outputs of the cascades, including those with large Hill coefficients.

AB - Background: MAPK cascades are signaling modules that function as switch generators. Results: In vitro phosphorylation in the p38 MAPK cascade tracked by LC-MS/MS revealed specific phosphorylation intermediates at each level. Conclusion: The p38 MAPK cascade reactions occur through intermediates MEK6/ST* and p38α/TY*. Significance: The precise order of reactions may contribute to the diverse kinetic outputs of the cascades, including those with large Hill coefficients.

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Precisely ordered phosphorylation reactions in the p38 mitogen-activated protein (MAP) kinase cascade

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