Potentiation of serum response factor activity by a family of myocardin-related transcription factors

Da Zhi Wang; Shijie Li; Dirk Hockemeyer; Lillian Sutherland; Zhigao Wang; Gerhard Schratt; James A. Richardson; Alfred Nordheim; Eric N. Olson

doi:10.1073/pnas.222561499

Potentiation of serum response factor activity by a family of myocardin-related transcription factors

Da Zhi Wang, Shijie Li, Dirk Hockemeyer, Lillian Sutherland, Zhigao Wang, Gerhard Schratt, James A. Richardson, Alfred Nordheim, Eric N. Olson

Research output: Contribution to journal › Article › peer-review

420 Scopus citations

Abstract

Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.

Original language	English (US)
Pages (from-to)	14855-14860
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Issue number	23
DOIs	https://doi.org/10.1073/pnas.222561499
State	Published - Nov 12 2002

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Wang, D. Z., Li, S., Hockemeyer, D., Sutherland, L., Wang, Z., Schratt, G., Richardson, J. A., Nordheim, A., & Olson, E. N. (2002). Potentiation of serum response factor activity by a family of myocardin-related transcription factors. Proceedings of the National Academy of Sciences of the United States of America, 99(23), 14855-14860. https://doi.org/10.1073/pnas.222561499

Wang, DZ, Li, S, Hockemeyer, D, Sutherland, L, Wang, Z, Schratt, G, Richardson, JA, Nordheim, A & Olson, EN 2002, 'Potentiation of serum response factor activity by a family of myocardin-related transcription factors', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 23, pp. 14855-14860. https://doi.org/10.1073/pnas.222561499

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abstract = "Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.",

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AU - Wang, Da Zhi

AU - Li, Shijie

AU - Hockemeyer, Dirk

AU - Sutherland, Lillian

AU - Wang, Zhigao

AU - Schratt, Gerhard

AU - Richardson, James A.

AU - Nordheim, Alfred

AU - Olson, Eric N.

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AB - Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.

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Potentiation of serum response factor activity by a family of myocardin-related transcription factors

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