@article{352152a8c4c4425cb49e0408fdd57630,
title = "Positional cloning of the mouse circadian Clock gene",
abstract = "We used positional cloning to identify the circadian Clock gene in mice. Clock is a large transcription unit with 24 exons spanning ~100,000 bp of DNA from which transcript classes of 7.5 and ~10 kb arise. Clock encodes a novel member of the bHLH-PAS family of transcription factors. In the Clock mutant allele, an A→T nucleotide transversion in a splice donor site causes exon skipping and deletion of 51 amino acids in the CLOCK protein. Clock is a unique gene with known circadian function and with features predicting DNA binding, protein dimerization, and activation domains. CLOCK represents the second example of a PAS domain-containing clock protein (besides Drosophila PERIOD), which suggests that this motif may define an evolutionarily conserved feature of the circadian clock mechanism.",
author = "King, {David P.} and Yaliang Zhao and Sangoram, {Ashvin M.} and Wilsbacher, {Lisa D.} and Minoru Tanaka and Antoch, {Marina P.} and Steeves, {Thomas D L} and Vitaterna, {Martha Hotz} and Kornhauser, {Jon M.} and Lowrey, {Phillip L.} and Turek, {Fred W.} and Takahashi, {Joseph S.}",
note = "Funding Information: Correspondence regarding this paper should be addressed to J. S. T. (e-mail: j-takahashi@nwu.edu). Special thanks are due to William F. Dove, Lawrence H. Pinto, Jeff Hall, Steve Kay, Michael Rosbash, and Michael Young for their advice and support. We thank our colleagues in the NSF Center for Biological Timing for their advice; Anne-Marie Chang and Genn Suyeoka for assistance with genetic mapping; Maja Bucan for Kit, Flk-1, and Pdgfra cDNA clones and for the yREB14 PCR primer sequences; Jeffrey M. Friedman and Eric Lander for advice on cloning strategies; Melvin Simon, Ung-Jin Kim, Nathaniel Heintz, Jian Zuo, and Philip De Jager for advice on BACs; Stephanie Chissoe and Cecilia Boyson for advice on shotgun sequencing of BACs; and Paul Hardin for unpublished information on per E box experiments. Research was supported by the NSF Center for Biological Timing, an Unrestricted Grant in Neuroscience from Bristol-Myers Squibb, NIMH grant R37 MH39592 and Northwestern University (J. S. T.), MSTP fellowship T32 GM08152 (L. D. W.), Japanese Ministry of Education fellowship (M. T.), and NIH P30 HD28048 (F. W. T.). ",
year = "1997",
month = may,
day = "16",
doi = "10.1016/S0092-8674(00)80245-7",
language = "English (US)",
volume = "89",
pages = "641--653",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",
}