Porcine nuclear transfer using somatic donor cells altered to express male germ cell function

Sangho Roh, Hye Yeon Choi, Sang Kyu Park, Cheolhee Won, Bong Woo Kim, Jung Hyun Kim, Hoin Kang, Eung Ryoung Lee, Ssang Goo Cho

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Recent studies reported that the direct transformation of one differentiated somatic cell type into another is possible. In the present study, we were able to modulate the cell fate of somatic cells to take on male germ cell function by introducing cell extracts derived from porcine testis tissue. Fibroblasts were treated with streptolysin O, which reversibly permeabilises the plasma membrane, and incubated with testis extracts. Our results showed that the testis extracts (TE) could activate expression of male germ cell-specific genes, implying that TE can provide regulatory components required for altering the cell fate of fibroblasts. Male germ cell function was sustained for more than 10 days after the introduction of TE. In addition, a single TE-treated cell was injected directly into the cytoplasm of in vitro-matured porcine oocytes. The rate of blastocyst formation was significantly higher in the TE-treated nuclear donor cell group than in the control cell group. The expression level of Nanog, Sox9 and Eomes was drastically increased when altered cells were used as donor nuclei. Our results suggest that TE can be used to alter the cell fate of fibroblasts to express male germ cell function and improve the developmental efficiency of the nuclear transfer porcine embryos.

Original languageEnglish (US)
Pages (from-to)882-891
Number of pages10
JournalReproduction, Fertility and Development
Issue number7
StatePublished - 2009
Externally publishedYes


  • Reprogramming
  • Testis extracts

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology


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