Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides

Ximing Zhou; Hasan K. Mubarak; Ling Ma; Doreen Palsgrove; Samuel Ortega; Gustavo Marrero Callicó; Edward A. Medina; Bradley B. Brimhall; Marisa Whitted; Baowei Fei

doi:10.1117/12.2655708

Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides

Ximing Zhou, Hasan K. Mubarak, Ling Ma, Doreen Palsgrove, Samuel Ortega, Gustavo Marrero Callicó, Edward A. Medina, Bradley B. Brimhall, Marisa Whitted, Baowei Fei

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

White blood cells, also called leukocytes, are hematopoietic cells of the immune system that are involved in protecting the body against both infectious diseases and foreign materials. The abnormal development and uncontrolled proliferation of these cells can lead to devastating cancers. Their timely recognition in the peripheral blood is critical to diagnosis and treatment. In this study, we developed a microscopic imaging system for improving the visualization of white blood cells on Wright's stained blood smear slides, with two different setups: polarized light imaging and polarized hyperspectral imaging. Based on the polarized light imaging setup, we collected the RGB images of Stokes vector parameters (S0, S1, S2, and S3) of five types of white blood cells (neutrophil, eosinophil, basophil, lymphocyte, and monocyte), and calculated the Stokes vector derived parameters: the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP)). We also calculated Stokes vector data based on the polarized hyperspectral imaging setup. The preliminary results demonstrate that Stokes vector derived parameters (DOP, DOLP, and DOCP) could improve the visualization of granules in granulocytes (neutrophils, eosinophils, and basophils). Furthermore, Stokes vector derived parameters (DOP, DOLP, and DOCP) could improve the visualization of surface structures (protein patterns) of lymphocytes enabling subclassification of lymphocyte subpopulations. Finally, S2, S3, and DOCP could enhance the morphologic visualization of monocyte nucleus. We also demonstrated that the polarized hyperspectral imaging setup could provide complementary spectral information to the spatial information on different Stokes vector parameters of white blood cells. This work demonstrates that polarized light imaging & polarized hyperspectral imaging has the potential to become a strong imaging tool in the diagnosis of disorders arising from white blood cells.

Original language	English (US)
Title of host publication	Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023
Editors	Jessica C. Ramella-Roman, Hui Ma, Tatiana Novikova, Daniel S. Elson, I. Alex Vitkin
Publisher	SPIE
ISBN (Electronic)	9781510658691
DOIs	https://doi.org/10.1117/12.2655708
State	Published - 2023
Event	Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023 - San Francisco, United States Duration: Jan 28 2023 → Jan 29 2023

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12382
ISSN (Print)	1605-7422

Conference

Conference	Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023
Country/Territory	United States
City	San Francisco
Period	1/28/23 → 1/29/23

Keywords

Hyperspectral imaging
Polarized hyperspectral imaging
Polarized light imaging
Stokes vector
White blood cell

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2655708

Cite this

Zhou, X., Mubarak, H. K., Ma, L., Palsgrove, D., Ortega, S., Callicó, G. M., Medina, E. A., Brimhall, B. B., Whitted, M., & Fei, B. (2023). Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides. In J. C. Ramella-Roman, H. Ma, T. Novikova, D. S. Elson, & I. A. Vitkin (Eds.), Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023 Article 1238207 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12382). SPIE. https://doi.org/10.1117/12.2655708

Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides. / Zhou, Ximing; Mubarak, Hasan K.; Ma, Ling et al.
Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023. ed. / Jessica C. Ramella-Roman; Hui Ma; Tatiana Novikova; Daniel S. Elson; I. Alex Vitkin. SPIE, 2023. 1238207 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12382).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Zhou, X, Mubarak, HK, Ma, L, Palsgrove, D, Ortega, S, Callicó, GM, Medina, EA, Brimhall, BB, Whitted, M & Fei, B 2023, Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides. in JC Ramella-Roman, H Ma, T Novikova, DS Elson & IA Vitkin (eds), Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023., 1238207, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12382, SPIE, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023, San Francisco, United States, 1/28/23. https://doi.org/10.1117/12.2655708

Zhou X, Mubarak HK, Ma L, Palsgrove D, Ortega S, Callicó GM et al. Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides. In Ramella-Roman JC, Ma H, Novikova T, Elson DS, Vitkin IA, editors, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023. SPIE. 2023. 1238207. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2655708

Zhou, Ximing ; Mubarak, Hasan K. ; Ma, Ling et al. / Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides. Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023. editor / Jessica C. Ramella-Roman ; Hui Ma ; Tatiana Novikova ; Daniel S. Elson ; I. Alex Vitkin. SPIE, 2023. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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title = "Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides",

abstract = "White blood cells, also called leukocytes, are hematopoietic cells of the immune system that are involved in protecting the body against both infectious diseases and foreign materials. The abnormal development and uncontrolled proliferation of these cells can lead to devastating cancers. Their timely recognition in the peripheral blood is critical to diagnosis and treatment. In this study, we developed a microscopic imaging system for improving the visualization of white blood cells on Wright's stained blood smear slides, with two different setups: polarized light imaging and polarized hyperspectral imaging. Based on the polarized light imaging setup, we collected the RGB images of Stokes vector parameters (S0, S1, S2, and S3) of five types of white blood cells (neutrophil, eosinophil, basophil, lymphocyte, and monocyte), and calculated the Stokes vector derived parameters: the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP)). We also calculated Stokes vector data based on the polarized hyperspectral imaging setup. The preliminary results demonstrate that Stokes vector derived parameters (DOP, DOLP, and DOCP) could improve the visualization of granules in granulocytes (neutrophils, eosinophils, and basophils). Furthermore, Stokes vector derived parameters (DOP, DOLP, and DOCP) could improve the visualization of surface structures (protein patterns) of lymphocytes enabling subclassification of lymphocyte subpopulations. Finally, S2, S3, and DOCP could enhance the morphologic visualization of monocyte nucleus. We also demonstrated that the polarized hyperspectral imaging setup could provide complementary spectral information to the spatial information on different Stokes vector parameters of white blood cells. This work demonstrates that polarized light imaging & polarized hyperspectral imaging has the potential to become a strong imaging tool in the diagnosis of disorders arising from white blood cells.",

keywords = "Hyperspectral imaging, Polarized hyperspectral imaging, Polarized light imaging, Stokes vector, White blood cell",

author = "Ximing Zhou and Mubarak, {Hasan K.} and Ling Ma and Doreen Palsgrove and Samuel Ortega and Callic{\'o}, {Gustavo Marrero} and Medina, {Edward A.} and Brimhall, {Bradley B.} and Marisa Whitted and Baowei Fei",

note = "Publisher Copyright: {\textcopyright} 2023 SPIE.; Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023 ; Conference date: 28-01-2023 Through 29-01-2023",

year = "2023",

doi = "10.1117/12.2655708",

language = "English (US)",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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T1 - Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides

AU - Zhou, Ximing

AU - Mubarak, Hasan K.

AU - Ma, Ling

AU - Palsgrove, Doreen

AU - Ortega, Samuel

AU - Callicó, Gustavo Marrero

AU - Medina, Edward A.

AU - Brimhall, Bradley B.

AU - Whitted, Marisa

AU - Fei, Baowei

PY - 2023

Y1 - 2023

N2 - White blood cells, also called leukocytes, are hematopoietic cells of the immune system that are involved in protecting the body against both infectious diseases and foreign materials. The abnormal development and uncontrolled proliferation of these cells can lead to devastating cancers. Their timely recognition in the peripheral blood is critical to diagnosis and treatment. In this study, we developed a microscopic imaging system for improving the visualization of white blood cells on Wright's stained blood smear slides, with two different setups: polarized light imaging and polarized hyperspectral imaging. Based on the polarized light imaging setup, we collected the RGB images of Stokes vector parameters (S0, S1, S2, and S3) of five types of white blood cells (neutrophil, eosinophil, basophil, lymphocyte, and monocyte), and calculated the Stokes vector derived parameters: the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP)). We also calculated Stokes vector data based on the polarized hyperspectral imaging setup. The preliminary results demonstrate that Stokes vector derived parameters (DOP, DOLP, and DOCP) could improve the visualization of granules in granulocytes (neutrophils, eosinophils, and basophils). Furthermore, Stokes vector derived parameters (DOP, DOLP, and DOCP) could improve the visualization of surface structures (protein patterns) of lymphocytes enabling subclassification of lymphocyte subpopulations. Finally, S2, S3, and DOCP could enhance the morphologic visualization of monocyte nucleus. We also demonstrated that the polarized hyperspectral imaging setup could provide complementary spectral information to the spatial information on different Stokes vector parameters of white blood cells. This work demonstrates that polarized light imaging & polarized hyperspectral imaging has the potential to become a strong imaging tool in the diagnosis of disorders arising from white blood cells.

AB - White blood cells, also called leukocytes, are hematopoietic cells of the immune system that are involved in protecting the body against both infectious diseases and foreign materials. The abnormal development and uncontrolled proliferation of these cells can lead to devastating cancers. Their timely recognition in the peripheral blood is critical to diagnosis and treatment. In this study, we developed a microscopic imaging system for improving the visualization of white blood cells on Wright's stained blood smear slides, with two different setups: polarized light imaging and polarized hyperspectral imaging. Based on the polarized light imaging setup, we collected the RGB images of Stokes vector parameters (S0, S1, S2, and S3) of five types of white blood cells (neutrophil, eosinophil, basophil, lymphocyte, and monocyte), and calculated the Stokes vector derived parameters: the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP)). We also calculated Stokes vector data based on the polarized hyperspectral imaging setup. The preliminary results demonstrate that Stokes vector derived parameters (DOP, DOLP, and DOCP) could improve the visualization of granules in granulocytes (neutrophils, eosinophils, and basophils). Furthermore, Stokes vector derived parameters (DOP, DOLP, and DOCP) could improve the visualization of surface structures (protein patterns) of lymphocytes enabling subclassification of lymphocyte subpopulations. Finally, S2, S3, and DOCP could enhance the morphologic visualization of monocyte nucleus. We also demonstrated that the polarized hyperspectral imaging setup could provide complementary spectral information to the spatial information on different Stokes vector parameters of white blood cells. This work demonstrates that polarized light imaging & polarized hyperspectral imaging has the potential to become a strong imaging tool in the diagnosis of disorders arising from white blood cells.

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KW - Stokes vector

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Y2 - 28 January 2023 through 29 January 2023

ER -

Polarized Hyperspectral Microscopic Imaging for White Blood Cells on Wright-Stained Blood Smear Slides

Abstract

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Keywords

ASJC Scopus subject areas

Access to Document

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