TY - GEN
T1 - Polarized hyperspectral microscopic imaging for collagen visualization on pathologic slides of head and neck squamous cell carcinoma
AU - Zhou, Ximing
AU - Mubarak, Hasan K.
AU - Ma, Ling
AU - Palsgrove, Doreen
AU - Sumer, Baran D.
AU - Fei, Baowei
N1 - Publisher Copyright:
© 2023 SPIE.
PY - 2023
Y1 - 2023
N2 - We developed a polarized hyperspectral microscope to collect four types of Stokes vector data cubes (S0, S1, S2, and S3) of the pathologic slides with head and neck squamous cell carcinoma (HNSCC). Our system consists of an optical light microscope with a movable stage, two polarizers, two liquid crystal variable retarders (LCVRs), and a SnapScan hyperspectral camera. The polarizers and LCVRs work in tandem with the hyperspectral camera to acquire polarized hyperspectral images. Synthetic pseudo-RGB images are generated from the four Stokes vector data cubes based on a transformation function similar to the spectral response of human eye for the visualization of hyperspectral images. Collagen is the most abundant extracellular matrix (ECM) protein in the human body. A major focus of studying the ECM in tumor microenvironment is the role of collagen in both normal and abnormal function. Collagen tends to accumulate in and around tumors during cancer development and growth. In this study, we acquired images from normal regions containing normal cells and collagen fibers and from tumor regions containing cancerous squamous cells and collagen fibers on HNSCC pathologic slides. The preliminary results demonstrated that our customized polarized hyperspectral microscope is able to improve the visualization of collagen on HNSCC pathologic slides under different situations, including thick fibers of normal stroma, thin fibers of normal stroma, fibers of normal muscle cells, fibers accumulated in tumors, fibers accumulated around tumors. Our preliminary results also demonstrated that the customized polarized hyperspectral microscope is capable of extracting the spectral signatures of collagen based on Stokes vector parameters and can have various applications in pathology and oncology.
AB - We developed a polarized hyperspectral microscope to collect four types of Stokes vector data cubes (S0, S1, S2, and S3) of the pathologic slides with head and neck squamous cell carcinoma (HNSCC). Our system consists of an optical light microscope with a movable stage, two polarizers, two liquid crystal variable retarders (LCVRs), and a SnapScan hyperspectral camera. The polarizers and LCVRs work in tandem with the hyperspectral camera to acquire polarized hyperspectral images. Synthetic pseudo-RGB images are generated from the four Stokes vector data cubes based on a transformation function similar to the spectral response of human eye for the visualization of hyperspectral images. Collagen is the most abundant extracellular matrix (ECM) protein in the human body. A major focus of studying the ECM in tumor microenvironment is the role of collagen in both normal and abnormal function. Collagen tends to accumulate in and around tumors during cancer development and growth. In this study, we acquired images from normal regions containing normal cells and collagen fibers and from tumor regions containing cancerous squamous cells and collagen fibers on HNSCC pathologic slides. The preliminary results demonstrated that our customized polarized hyperspectral microscope is able to improve the visualization of collagen on HNSCC pathologic slides under different situations, including thick fibers of normal stroma, thin fibers of normal stroma, fibers of normal muscle cells, fibers accumulated in tumors, fibers accumulated around tumors. Our preliminary results also demonstrated that the customized polarized hyperspectral microscope is capable of extracting the spectral signatures of collagen based on Stokes vector parameters and can have various applications in pathology and oncology.
KW - Hyperspectral imaging
KW - Stokes vector
KW - collagen
KW - head and neck cancer
KW - polarized hyperspectral imaging
KW - polarized light imaging
UR - http://www.scopus.com/inward/record.url?scp=85159781495&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85159781495&partnerID=8YFLogxK
U2 - 10.1117/12.2655831
DO - 10.1117/12.2655831
M3 - Conference contribution
C2 - 38481487
AN - SCOPUS:85159781495
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023
A2 - Ramella-Roman, Jessica C.
A2 - Ma, Hui
A2 - Novikova, Tatiana
A2 - Elson, Daniel S.
A2 - Vitkin, I. Alex
PB - SPIE
T2 - Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023
Y2 - 28 January 2023 through 29 January 2023
ER -