Photoactivatable and water soluble FRET dyes with high uncaging cross section

Genhua Zheng; Yan Ming Guo; Wen Hong Li

doi:10.1021/ja071427+

Photoactivatable and water soluble FRET dyes with high uncaging cross section

Genhua Zheng, Yan Ming Guo, Wen Hong Li

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Photoactivatable fluorophores are powerful imaging probes for tracking molecular and cellular dynamics with high spatiotemporal resolution. We have designed and synthesized a new caged FRET dye, CCC-1, comprising a caged coumarin and a water soluble xanthene dye. CCC-1 can be efficiently photolyzed by ultraviolet light. Its photolyzed product, CC-1, emits strong green fluorescence with a FRET efficiency of 95% when excited near 410 nm. The uncaging cross section of CCC-1 is about 6000 M^-1cm^-1 at 365 nm, more than a hundred times higher than caged fluoresceins developed previously. The probe is also biocompatible and has built-in functionalities for bioconjugation.

Original language	English (US)
Pages (from-to)	10616-10617
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	129
Issue number	35
DOIs	https://doi.org/10.1021/ja071427+
State	Published - Sep 5 2007

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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abstract = "Photoactivatable fluorophores are powerful imaging probes for tracking molecular and cellular dynamics with high spatiotemporal resolution. We have designed and synthesized a new caged FRET dye, CCC-1, comprising a caged coumarin and a water soluble xanthene dye. CCC-1 can be efficiently photolyzed by ultraviolet light. Its photolyzed product, CC-1, emits strong green fluorescence with a FRET efficiency of 95% when excited near 410 nm. The uncaging cross section of CCC-1 is about 6000 M-1cm-1 at 365 nm, more than a hundred times higher than caged fluoresceins developed previously. The probe is also biocompatible and has built-in functionalities for bioconjugation.",

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AB - Photoactivatable fluorophores are powerful imaging probes for tracking molecular and cellular dynamics with high spatiotemporal resolution. We have designed and synthesized a new caged FRET dye, CCC-1, comprising a caged coumarin and a water soluble xanthene dye. CCC-1 can be efficiently photolyzed by ultraviolet light. Its photolyzed product, CC-1, emits strong green fluorescence with a FRET efficiency of 95% when excited near 410 nm. The uncaging cross section of CCC-1 is about 6000 M-1cm-1 at 365 nm, more than a hundred times higher than caged fluoresceins developed previously. The probe is also biocompatible and has built-in functionalities for bioconjugation.

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Photoactivatable and water soluble FRET dyes with high uncaging cross section

Abstract

ASJC Scopus subject areas

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