Peripheral tolerance through clonal deletion of mature CD4-CD8+ T cells

Douglas A. Carlow, Soo Jeet Teh, Nicolai S C Van Oers, Richard G. Miller, Hung sia Teh

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Transgenic mice bearing the αβ transgenes encoding a defined T cell receptor specific for the male (H-Y) antigen presented by the H-2Db class I MHC molecule were used to study mechanisms of peripheral tolerance. Female transgenic mice produce large numbers of functionally homogeneous CD8+ male antigen-reactive T cells in the thymus that subsequently accumulate In the peripheral lymphoid organs. We have used three experimental approaches to show that male reactive CD8+ T cells can be eliminated from peripheral lymphoid organs after exposure to male antigen, (i) In female transgenic mice that were neonatally tolerized with male spleen cells, male reactive CD8+ T cells continued to be produced in large numbers In the thymus but were virtually absent in the lymph nodes. (II) Injection of thymocytes from female transgenic mice Into female mice neonatally tolerized with the male antigen, or Into normal male mice, led to the specific elimination of male-reactive CD8+ T cells in the lymph nodes, (iii) Four days after male lymphoid cells were Injected intravenously into female transgenic mice, male antigen-reactive CD8+ T cells recovered from the lymph nodes of recipient mice were highly apoptotlc when compared to CD4+ (non-male reactive) T cells. These data indicate that tolerance to extrathymic antigen can be achieved through elimination of mature T cells in the peripheral lymphoid organs.

Original languageEnglish (US)
Pages (from-to)599-610
Number of pages12
JournalInternational Immunology
Issue number5
StatePublished - May 1992


  • Extrathymic deletion
  • Extrathymic tolerance
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Peripheral tolerance through clonal deletion of mature CD4-CD8+ T cells'. Together they form a unique fingerprint.

Cite this