Peptide-conjugated quantum dots activate neuronal receptors and initiate downstream signaling of neurite growth

Tania Q. Vu; Ravikanth Maddipati; Todd A. Blute; Barrett J. Nehilla; Leora Nusblat; Tejal A. Desai

doi:10.1021/nl047977c

Peptide-conjugated quantum dots activate neuronal receptors and initiate downstream signaling of neurite growth

Tania Q. Vu, Ravikanth Maddipati, Todd A. Blute, Barrett J. Nehilla, Leora Nusblat, Tejal A. Desai

Research output: Contribution to journal › Article › peer-review

126 Scopus citations

Abstract

Quantum dots (QDs) could serve as fluorescent scaffolds for effecting specific physiological and pharmacological responses in cells. Here, we conjugate the peptide ligand βNGF to QD surfaces, and confirm surface modification and single QD nanostructure using AFM. We show that βNGF-QDs retain bioactivity, activate TrkA receptors, and initiate neuronal differentiation in PC12 cells. Receptor-evoked activity of QD-immobilized ligands has wide-ranging implications for the development of molecular tools and therapeutics targeted at understanding and regulating cell function.

Original language	English (US)
Pages (from-to)	603-607
Number of pages	5
Journal	Nano Letters
Volume	5
Issue number	4
DOIs	https://doi.org/10.1021/nl047977c
State	Published - Apr 2005
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl047977c

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abstract = "Quantum dots (QDs) could serve as fluorescent scaffolds for effecting specific physiological and pharmacological responses in cells. Here, we conjugate the peptide ligand βNGF to QD surfaces, and confirm surface modification and single QD nanostructure using AFM. We show that βNGF-QDs retain bioactivity, activate TrkA receptors, and initiate neuronal differentiation in PC12 cells. Receptor-evoked activity of QD-immobilized ligands has wide-ranging implications for the development of molecular tools and therapeutics targeted at understanding and regulating cell function.",

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AU - Vu, Tania Q.

AU - Maddipati, Ravikanth

AU - Blute, Todd A.

AU - Nehilla, Barrett J.

AU - Nusblat, Leora

AU - Desai, Tejal A.

PY - 2005/4

Y1 - 2005/4

N2 - Quantum dots (QDs) could serve as fluorescent scaffolds for effecting specific physiological and pharmacological responses in cells. Here, we conjugate the peptide ligand βNGF to QD surfaces, and confirm surface modification and single QD nanostructure using AFM. We show that βNGF-QDs retain bioactivity, activate TrkA receptors, and initiate neuronal differentiation in PC12 cells. Receptor-evoked activity of QD-immobilized ligands has wide-ranging implications for the development of molecular tools and therapeutics targeted at understanding and regulating cell function.

AB - Quantum dots (QDs) could serve as fluorescent scaffolds for effecting specific physiological and pharmacological responses in cells. Here, we conjugate the peptide ligand βNGF to QD surfaces, and confirm surface modification and single QD nanostructure using AFM. We show that βNGF-QDs retain bioactivity, activate TrkA receptors, and initiate neuronal differentiation in PC12 cells. Receptor-evoked activity of QD-immobilized ligands has wide-ranging implications for the development of molecular tools and therapeutics targeted at understanding and regulating cell function.

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Peptide-conjugated quantum dots activate neuronal receptors and initiate downstream signaling of neurite growth

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