TY - JOUR
T1 - Pentobarbital modifies the lipid raft-protein interaction
T2 - A first clue about the anesthesia mechanism on NMDA and GABAA receptors
AU - Sierra-Valdez, Francisco Javier
AU - Ruiz-Suárez, J. C.
AU - Delint-Ramirez, Ilse
N1 - Funding Information:
This work was supported by Conacyt, México , under Grants 180919 and 220962 .
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - Recent studies have shown that anesthetic agents alter the physical properties of lipid rafts on model membranes. However, if this destabilization occurs in brain membranes, altering the lipid raft-protein interaction, remains unknown. We analyzed the effects produced by pentobarbital (PB) on brain plasma membranes and lipid rafts in vivo. We characterized for the first time the thermotropic behavior of plasma membranes, synaptosomes, and lipid rafts from rat brain. We found that the transition temperature from the ordered gel to disordered liquid phase of lipids is close to physiological temperature. We then studied the effect of PB on protein composition of lipid rafts. Our results show a reduction of the total protein associated to rafts, with a higher reduction of the NMDAR compared to the GABA A receptor. Both receptors are considered the main targets of PB. In general, our results suggest that lipid rafts could be plausible mediators in anesthetic action.
AB - Recent studies have shown that anesthetic agents alter the physical properties of lipid rafts on model membranes. However, if this destabilization occurs in brain membranes, altering the lipid raft-protein interaction, remains unknown. We analyzed the effects produced by pentobarbital (PB) on brain plasma membranes and lipid rafts in vivo. We characterized for the first time the thermotropic behavior of plasma membranes, synaptosomes, and lipid rafts from rat brain. We found that the transition temperature from the ordered gel to disordered liquid phase of lipids is close to physiological temperature. We then studied the effect of PB on protein composition of lipid rafts. Our results show a reduction of the total protein associated to rafts, with a higher reduction of the NMDAR compared to the GABA A receptor. Both receptors are considered the main targets of PB. In general, our results suggest that lipid rafts could be plausible mediators in anesthetic action.
KW - Anesthesia
KW - Calorimetry
KW - Lipid membrane
KW - Receptors
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U2 - 10.1016/j.bbamem.2016.07.011
DO - 10.1016/j.bbamem.2016.07.011
M3 - Article
C2 - 27457704
AN - SCOPUS:84982719241
SN - 0005-2736
VL - 1858
SP - 2603
EP - 2610
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 11
ER -