PCEST: Positive contrast using Chemical Exchange Saturation Transfer

Elena Vinogradov, Todd C. Soesbe, James A. Balschi, A. Dean Sherry, Robert E. Lenkinski

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Chemical Exchange Saturation Transfer (CEST) contrast utilizes selective pre-saturation of a small pool of exchanging protons and subsequent detection of the decrease in bulk water signal. The CEST contrast is negative and requires detection of small signal change in the presence of a strong background signal. Here we develop a Positive CEST (pCEST) detection scheme utilizing the analogous nature of the CEST and off-resonance T experiments and exploring increased apparent relaxation rates in the presence of the selective pre-saturation. pCEST leads to the positive contrast, i.e., increased signal intensity as the result of the presence of the agent and RF pre-saturation. Simultaneously substantial background suppression is achieved. The contrast can be switched "ON" and "OFF", similar to the original CEST.

Original languageEnglish (US)
Pages (from-to)64-73
Number of pages10
JournalJournal of Magnetic Resonance
StatePublished - Feb 2011


  • CEST
  • Off-resonance T
  • Positive CEST

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics


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