Pathogenic orphan transduction created by a nonreference LINE-1 retrotransposon

Szilvia Solyom, Adam D. Ewing, Dustin C. Hancks, Yasuhiro Takeshima, Hiroyuki Awano, Masafumi Matsuo, Haig H. Kazazian

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Long INterspersed Element-1 (LINE-1) retrotransposons comprise 17% of the human genome, and move by a potentially mutagenic "copy and paste" mechanism via an RNA intermediate. Recently, the retrotransposition-mediated insertion of a new transcript was described as a novel cause of genetic disease, Duchenne muscular dystrophy, in a Japanese male. The inserted sequence was presumed to derive from a single-copy, noncoding RNA transcribed from chromosome 11q22.3 that retrotransposed into the dystrophin gene. Here, we demonstrate that a nonreference fulllength LINE-1 is situated in the proband and maternal genome at chromosome 11q22.3, directly upstream of the sequence, whose copy was inserted into the dystrophin gene. This LINE-1 is highly active in a cell culture assay. LINE-1 insertions are often associated with 3' transduction of adjacent genomic sequences. Thus, the likely explanation for the mutagenic insertion is a LINE-1-mediated 3' transduction with severe 5' truncation. This is the first example of LINE-1-induced human disease caused by an "orphan" 3' transduction.

Original languageEnglish (US)
Pages (from-to)369-371
Number of pages3
JournalHuman mutation
Issue number2
StatePublished - Feb 2012


  • 3'transduction
  • Duchenne muscular dystrophy
  • Dystrophin
  • Line-1
  • Retrotransposon

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)


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