Abstract
Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.
Original language | English (US) |
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Pages (from-to) | 2114-2117 |
Number of pages | 4 |
Journal | Molecular BioSystems |
Volume | 7 |
Issue number | 7 |
DOIs | |
State | Published - Jul 1 2011 |
ASJC Scopus subject areas
- Biotechnology
- Molecular Biology