Optogenetic research of hippocampal neuron activity in vitro in the ps1-m146v mouse model of Alzheimer's disease

A. I. Erofeev; O. A. Zakharova; S. G. Terekhin; P. V. Plotnikova; I. B. Bezprozvanny; O. L. Vlasova

doi:10.7868/S0044467717050070

Optogenetic research of hippocampal neuron activity in vitro in the ps1-m146v mouse model of Alzheimer's disease

A. I. Erofeev, O. A. Zakharova, S. G. Terekhin, P. V. Plotnikova, I. B. Bezprozvanny, O. L. Vlasova

Research output: Contribution to journal › Review article › peer-review

Abstract

In this paper we gave a comparative study of the various physico-chemical properties of the hippocampal neurons in cell culture model of Alzheimer's Desease (Presenilin 1 mutations). It is shown that chemical, electrical and optical effects increase neuronal excitability differently for wild-Type and KI (PS 1-M146V neurons). In contrast to wild type KI neurons were more excitable, especially at the beginning of stimulation, but over time, this activity has been decreasing. At the same time, only in the case of optogenetic stimulation, exceeding the absolute values of their numbers has been registered, which made it possible to reduce their effect on the corresponding indices in wild type neurons. Molecular-cellular mechanisms of this phenomenon can serve as a material for further studies, both fundamental and practical (determination of the regimes of regulation of the electrical activity of neurons).

Original language	English (US)
Pages (from-to)	63-74
Number of pages	12
Journal	Zhurnal Vysshei Nervnoi Deyatelnosti Imeni I.P. Pavlova
Volume	67
Issue number	5
DOIs	https://doi.org/10.7868/S0044467717050070
State	Published - Jan 1 2017

Keywords

Alzheimer's disease
Hippocampal neurons
Optogenetics

ASJC Scopus subject areas

General Neuroscience

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10.7868/S0044467717050070

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title = "Optogenetic research of hippocampal neuron activity in vitro in the ps1-m146v mouse model of Alzheimer's disease",

abstract = "In this paper we gave a comparative study of the various physico-chemical properties of the hippocampal neurons in cell culture model of Alzheimer's Desease (Presenilin 1 mutations). It is shown that chemical, electrical and optical effects increase neuronal excitability differently for wild-Type and KI (PS 1-M146V neurons). In contrast to wild type KI neurons were more excitable, especially at the beginning of stimulation, but over time, this activity has been decreasing. At the same time, only in the case of optogenetic stimulation, exceeding the absolute values of their numbers has been registered, which made it possible to reduce their effect on the corresponding indices in wild type neurons. Molecular-cellular mechanisms of this phenomenon can serve as a material for further studies, both fundamental and practical (determination of the regimes of regulation of the electrical activity of neurons).",

keywords = "Alzheimer's disease, Hippocampal neurons, Optogenetics",

author = "Erofeev, {A. I.} and Zakharova, {O. A.} and Terekhin, {S. G.} and Plotnikova, {P. V.} and Bezprozvanny, {I. B.} and Vlasova, {O. L.}",

year = "2017",

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doi = "10.7868/S0044467717050070",

language = "English (US)",

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journal = "Zhurnal Vysshei Nervnoi Deyatelnosti Imeni I.P. Pavlova",

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T1 - Optogenetic research of hippocampal neuron activity in vitro in the ps1-m146v mouse model of Alzheimer's disease

AU - Erofeev, A. I.

AU - Zakharova, O. A.

AU - Terekhin, S. G.

AU - Plotnikova, P. V.

AU - Bezprozvanny, I. B.

AU - Vlasova, O. L.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - In this paper we gave a comparative study of the various physico-chemical properties of the hippocampal neurons in cell culture model of Alzheimer's Desease (Presenilin 1 mutations). It is shown that chemical, electrical and optical effects increase neuronal excitability differently for wild-Type and KI (PS 1-M146V neurons). In contrast to wild type KI neurons were more excitable, especially at the beginning of stimulation, but over time, this activity has been decreasing. At the same time, only in the case of optogenetic stimulation, exceeding the absolute values of their numbers has been registered, which made it possible to reduce their effect on the corresponding indices in wild type neurons. Molecular-cellular mechanisms of this phenomenon can serve as a material for further studies, both fundamental and practical (determination of the regimes of regulation of the electrical activity of neurons).

AB - In this paper we gave a comparative study of the various physico-chemical properties of the hippocampal neurons in cell culture model of Alzheimer's Desease (Presenilin 1 mutations). It is shown that chemical, electrical and optical effects increase neuronal excitability differently for wild-Type and KI (PS 1-M146V neurons). In contrast to wild type KI neurons were more excitable, especially at the beginning of stimulation, but over time, this activity has been decreasing. At the same time, only in the case of optogenetic stimulation, exceeding the absolute values of their numbers has been registered, which made it possible to reduce their effect on the corresponding indices in wild type neurons. Molecular-cellular mechanisms of this phenomenon can serve as a material for further studies, both fundamental and practical (determination of the regimes of regulation of the electrical activity of neurons).

KW - Alzheimer's disease

KW - Hippocampal neurons

KW - Optogenetics

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Optogenetic research of hippocampal neuron activity in vitro in the ps1-m146v mouse model of Alzheimer's disease

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Keywords

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