Optogenetic control of ATP release

Matthew A. Lewis, Bipin Joshi, Ling Gu, Andrew Feranchak, Samarendra K. Mohanty

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Controlled release of ATP can be used for understanding extracellular purinergic signaling. While coarse mechanical forces and hypotonic stimulation have been utilized in the past to initiate ATP release from cells, these methods are neither spatially accurate nor temporally precise. Further, these methods cannot be utilized in a highly effective cell-specific manner. To mitigate the uncertainties regarding cellular-specificity and spatio-temporal release of ATP, we herein demonstrate use of optogenetics for ATP release. ATP release in response to optogenetic stimulation was monitored by Luciferin-Luciferase assay (North American firefly, photinus pyralis) using luminometer as well as mesoscopic bioluminescence imaging. Our result demonstrates repetitive release of ATP subsequent to optogenetic stimulation. It is thus feasible that purinergic signaling can be directly detected via imaging if the stimulus can be confined to single cell or in a spatially-defined group of cells. This study opens up new avenue to interrogate the mechanisms of purinergic signaling.

Original languageEnglish (US)
Title of host publicationOptogenetics
Subtitle of host publicationOptical Methods for Cellular Control
StatePublished - 2013
EventOptogenetics: Optical Methods for Cellular Control - San Francisco, CA, United States
Duration: Feb 2 2013Feb 3 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherOptogenetics: Optical Methods for Cellular Control
Country/TerritoryUnited States
CitySan Francisco, CA


  • ATP release
  • Chemiluminescence
  • Mesoscopic imaging
  • Optogenetics stimulation
  • Purinergic signaling

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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