TY - JOUR
T1 - OMNY PIN - A versatile sample holder for tomographic measurements at room and cryogenic temperatures
AU - Holler, M.
AU - Raabe, J.
AU - Wepf, R.
AU - Shahmoradian, S. H.
AU - Diaz, A.
AU - Sarafimov, B.
AU - Lachat, T.
AU - Walther, H.
AU - Vitins, M.
N1 - Funding Information:
We thank ScopeM, the scientific center for optical and electron microscopy of ETH Zürich, for providing access to the xenon FIB/SEM (Tescan, Fera3) and Takashi Ishikawa and Dennis R. Diener for providing the Chlamydomonas samples. This work was supported by the SNF R’Equip (Project No. 145056). Ptychography data were collected at the coherent small-angle x-ray scattering (cSAXS) beamline at the Swiss Light Source at the Paul Scherrer Institut in Villigen, Switzerland, using the OMNY instrument.
Publisher Copyright:
© 2017 Author(s).
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Nowadays ptychographic tomography in the hard x-ray regime, i.e., at energies above about 2 keV, is a well-established measurement technique. At the Paul Scherrer Institut, currently two instruments are available: one is measuring at room temperature and atmospheric pressure, and the other, the so-called OMNY (tOMography Nano crYo) instrument, is operating at ultra-high vacuum and offering cryogenic sample temperatures down to 10 K. In this manuscript, we present the sample mounts that were developed for these instruments. Aside from excellent mechanical stability and thermal conductivity, they also offer highly reproducible mounting. Various types were developed for different kinds of samples and are presented in detail, including examples of how specimens can be mounted on these holders. We also show the first hard x-ray ptychographic tomography measurements of high-pressure frozen biological samples, in the present case Chlamydomonas cells, the related sample pins and preparation steps. For completeness, we present accessories such as transportation containers for both room temperature and cryogenic samples and a gripper mechanism for automatic sample changing. The sample mounts are not limited to x-ray tomography or hard x-ray energies, and we believe that they can be very useful for other instrumentation projects.
AB - Nowadays ptychographic tomography in the hard x-ray regime, i.e., at energies above about 2 keV, is a well-established measurement technique. At the Paul Scherrer Institut, currently two instruments are available: one is measuring at room temperature and atmospheric pressure, and the other, the so-called OMNY (tOMography Nano crYo) instrument, is operating at ultra-high vacuum and offering cryogenic sample temperatures down to 10 K. In this manuscript, we present the sample mounts that were developed for these instruments. Aside from excellent mechanical stability and thermal conductivity, they also offer highly reproducible mounting. Various types were developed for different kinds of samples and are presented in detail, including examples of how specimens can be mounted on these holders. We also show the first hard x-ray ptychographic tomography measurements of high-pressure frozen biological samples, in the present case Chlamydomonas cells, the related sample pins and preparation steps. For completeness, we present accessories such as transportation containers for both room temperature and cryogenic samples and a gripper mechanism for automatic sample changing. The sample mounts are not limited to x-ray tomography or hard x-ray energies, and we believe that they can be very useful for other instrumentation projects.
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U2 - 10.1063/1.4996092
DO - 10.1063/1.4996092
M3 - Article
C2 - 29195351
AN - SCOPUS:85035007266
SN - 0034-6748
VL - 88
JO - Review of Scientific Instruments
JF - Review of Scientific Instruments
IS - 11
M1 - 113701
ER -