Novel higher-order epigenetic regulation of the Bdnf gene upon seizures

Agnieszka Walczak, Andrzej A. Szczepankiewicz, Blazej Ruszczycki, Adriana Magalska, Katarzyna Zamlynska, Joanna Dzwonek, Ewa Wilczek, Katarzyna Zybura-Broda, Marcin Rylski, Monika Malinowska, Michal Dabrowski, Teresa Szczepinska, Krzysztof Pawlowski, Marta Pyskaty, Jakub Wlodarczyk, Izabela Szczerbal, Marek Switonski, Marion Cremer, Grzegorz M. Wilczynski

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


Studies in cultured cells have demonstrated the existence of higher-order epigenetic mechanisms, determining the relationship between expression of the gene and its position within the cell nucleus. It is unknown, whether such mechanisms operate in postmitotic, highly differentiated cell types, such as neurons in vivo. Accordingly, we examined whether the intranuclear positions of Bdnf and Trkb genes, encoding the major neurotrophin and its receptor respectively, change as a result of neuronal activity, and what functional consequences such movements may have. In a rat model of massive neuronal activation upon kainate-induced seizures we found that elevated neuronal expression of Bdnf is associated with its detachment from the nuclear lamina, and translocation toward the nucleus center. In contrast, the position of stably expressed Trkb remains unchanged after seizures. Our study demonstrates that activation-dependent architectural remodeling of the neuronal cell nucleus in vivo contributes to activity-dependent changes in gene expression in the brain.

Original languageEnglish (US)
Pages (from-to)2507-2511
Number of pages5
JournalJournal of Neuroscience
Issue number6
StatePublished - Feb 6 2013
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)


Dive into the research topics of 'Novel higher-order epigenetic regulation of the Bdnf gene upon seizures'. Together they form a unique fingerprint.

Cite this