Noninvasive evaluation of liver metabolism by 2H and 13C NMR isotopomer analysis of human urine

Shawn C. Burgess; Brian Weis; John G. Jones; Erin Smith; Matthew E. Merritt; David Margolis; A. Dean Sherry; Craig R. Malloy

doi:10.1016/S0003-2697(02)00465-7

Noninvasive evaluation of liver metabolism by ²H and ¹³C NMR isotopomer analysis of human urine

Shawn C. Burgess, Brian Weis, John G. Jones, Erin Smith, Matthew E. Merritt, David Margolis, A. Dean Sherry, Craig R. Malloy

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Mammalian liver disposes of acetaminophen and other ingested xenobiotics by forming soluble glucuronides that are subsequently removed via renal filtration. When given in combination with the stable isotopes ²H and ¹³C, the glucuronide of acetaminophen isolated from urine provides a convenient "chemical biopsy" for evaluating intermediary metabolism in the liver. Here, we describe isolation and purification of urinary acetaminophen glucuronide and its conversion to monoacetone glucose (MAG). Subsequent ²H and ¹³C NMR analysis of MAG from normal volunteers after ingestion of ²H₂O and [U-¹³C₃]propionate allowed a noninvasive profiling of hepatic gluconeogenic pathways. The method should find use in metabolic studies of infants and other populations where blood sampling is either limited or problematic.

Original language	English (US)
Pages (from-to)	228-234
Number of pages	7
Journal	Analytical biochemistry
Volume	312
Issue number	2
DOIs	https://doi.org/10.1016/S0003-2697(02)00465-7
State	Published - Jan 15 2003

Keywords

C-isotopomer analysis
Citric acid cycle
Gluconeogenesis
Glycogenolysis
H NMR
Urine

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0003-2697(02)00465-7

Cite this

@article{0188f31d0e524fdfade3a4474eb08a1b,

title = "Noninvasive evaluation of liver metabolism by 2H and 13C NMR isotopomer analysis of human urine",

abstract = "Mammalian liver disposes of acetaminophen and other ingested xenobiotics by forming soluble glucuronides that are subsequently removed via renal filtration. When given in combination with the stable isotopes 2H and 13C, the glucuronide of acetaminophen isolated from urine provides a convenient {"}chemical biopsy{"} for evaluating intermediary metabolism in the liver. Here, we describe isolation and purification of urinary acetaminophen glucuronide and its conversion to monoacetone glucose (MAG). Subsequent 2H and 13C NMR analysis of MAG from normal volunteers after ingestion of 2H2O and [U-13C3]propionate allowed a noninvasive profiling of hepatic gluconeogenic pathways. The method should find use in metabolic studies of infants and other populations where blood sampling is either limited or problematic.",

keywords = "C-isotopomer analysis, Citric acid cycle, Gluconeogenesis, Glycogenolysis, H NMR, Urine",

author = "Burgess, {Shawn C.} and Brian Weis and Jones, {John G.} and Erin Smith and Merritt, {Matthew E.} and David Margolis and Sherry, {A. Dean} and Malloy, {Craig R.}",

note = "Funding Information: This work was supported by NIH Grants P41-RR02584 and HL34557. We thank Liwei Cao, Ph.D., for technical assistance and Holly Wise for study coordination. ",

year = "2003",

month = jan,

day = "15",

doi = "10.1016/S0003-2697(02)00465-7",

language = "English (US)",

volume = "312",

pages = "228--234",

journal = "Analytical biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Noninvasive evaluation of liver metabolism by 2H and 13C NMR isotopomer analysis of human urine

AU - Burgess, Shawn C.

AU - Weis, Brian

AU - Jones, John G.

AU - Smith, Erin

AU - Merritt, Matthew E.

AU - Margolis, David

AU - Sherry, A. Dean

AU - Malloy, Craig R.

N1 - Funding Information: This work was supported by NIH Grants P41-RR02584 and HL34557. We thank Liwei Cao, Ph.D., for technical assistance and Holly Wise for study coordination.

PY - 2003/1/15

Y1 - 2003/1/15

N2 - Mammalian liver disposes of acetaminophen and other ingested xenobiotics by forming soluble glucuronides that are subsequently removed via renal filtration. When given in combination with the stable isotopes 2H and 13C, the glucuronide of acetaminophen isolated from urine provides a convenient "chemical biopsy" for evaluating intermediary metabolism in the liver. Here, we describe isolation and purification of urinary acetaminophen glucuronide and its conversion to monoacetone glucose (MAG). Subsequent 2H and 13C NMR analysis of MAG from normal volunteers after ingestion of 2H2O and [U-13C3]propionate allowed a noninvasive profiling of hepatic gluconeogenic pathways. The method should find use in metabolic studies of infants and other populations where blood sampling is either limited or problematic.

AB - Mammalian liver disposes of acetaminophen and other ingested xenobiotics by forming soluble glucuronides that are subsequently removed via renal filtration. When given in combination with the stable isotopes 2H and 13C, the glucuronide of acetaminophen isolated from urine provides a convenient "chemical biopsy" for evaluating intermediary metabolism in the liver. Here, we describe isolation and purification of urinary acetaminophen glucuronide and its conversion to monoacetone glucose (MAG). Subsequent 2H and 13C NMR analysis of MAG from normal volunteers after ingestion of 2H2O and [U-13C3]propionate allowed a noninvasive profiling of hepatic gluconeogenic pathways. The method should find use in metabolic studies of infants and other populations where blood sampling is either limited or problematic.

KW - C-isotopomer analysis

KW - Citric acid cycle

KW - Gluconeogenesis

KW - Glycogenolysis

KW - H NMR

KW - Urine

UR - http://www.scopus.com/inward/record.url?scp=0037439386&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037439386&partnerID=8YFLogxK

U2 - 10.1016/S0003-2697(02)00465-7

DO - 10.1016/S0003-2697(02)00465-7

M3 - Article

C2 - 12531210

AN - SCOPUS:0037439386

SN - 0003-2697

VL - 312

SP - 228

EP - 234

JO - Analytical biochemistry

JF - Analytical biochemistry

IS - 2

ER -