New directions for Fluorescent Speckle Microscopy

Clare M. Waterman-Storer; Gaudenz Danuser

doi:10.1016/S0960-9822(02)01139-9

New directions for Fluorescent Speckle Microscopy

Clare M. Waterman-Storer, Gaudenz Danuser

Research output: Contribution to journal › Review article › peer-review

40 Scopus citations

Abstract

Fluorescent Speckle Microscopy (FSM) is a technology for analyzing cytoskeleton dynamics, giving novel insight into their roles in living cells. New applications of FSM, together with the development of computer-based FSM image analysis, will make FSM the first microscopy-based method to deliver quantitative kinetic readouts at high spatial and temporal resolution for a wide variety of macromolecular systems. Here, we review the most recent applications and developments and give a glimpse of future directions and potentials of FSM.

Original language	English (US)
Pages (from-to)	R633-R640
Journal	Current Biology
Volume	12
Issue number	18
DOIs	https://doi.org/10.1016/S0960-9822(02)01139-9
State	Published - Sep 17 2002

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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10.1016/S0960-9822(02)01139-9

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title = "New directions for Fluorescent Speckle Microscopy",

abstract = "Fluorescent Speckle Microscopy (FSM) is a technology for analyzing cytoskeleton dynamics, giving novel insight into their roles in living cells. New applications of FSM, together with the development of computer-based FSM image analysis, will make FSM the first microscopy-based method to deliver quantitative kinetic readouts at high spatial and temporal resolution for a wide variety of macromolecular systems. Here, we review the most recent applications and developments and give a glimpse of future directions and potentials of FSM.",

author = "Waterman-Storer, {Clare M.} and Gaudenz Danuser",

note = "Funding Information: Supported by NIH GM 61804-01 to CMWS, SNF 21-59452.99 to GD, and a collaborative Young Investigators Award from the Human Frontiers Science Programme to C.M.W.-S., G.D., and Inke N{\"a}thke (University of Dundee). The authors gratefully acknowledge A. Ponti and P. Vallotton for programming the algorithms for FSM analysis, Nikon Inc, for the use of the TIRF system, and Ted Salmon, Paul Maddox, Julie Canman, Chris Cohan, Ingrid Brust-Mascher, and Jon Scholey for sharing unpublished data.",

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N2 - Fluorescent Speckle Microscopy (FSM) is a technology for analyzing cytoskeleton dynamics, giving novel insight into their roles in living cells. New applications of FSM, together with the development of computer-based FSM image analysis, will make FSM the first microscopy-based method to deliver quantitative kinetic readouts at high spatial and temporal resolution for a wide variety of macromolecular systems. Here, we review the most recent applications and developments and give a glimpse of future directions and potentials of FSM.

AB - Fluorescent Speckle Microscopy (FSM) is a technology for analyzing cytoskeleton dynamics, giving novel insight into their roles in living cells. New applications of FSM, together with the development of computer-based FSM image analysis, will make FSM the first microscopy-based method to deliver quantitative kinetic readouts at high spatial and temporal resolution for a wide variety of macromolecular systems. Here, we review the most recent applications and developments and give a glimpse of future directions and potentials of FSM.

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New directions for Fluorescent Speckle Microscopy

Abstract

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