Multiple splice forms of ribonuclease-inhibitor mRNA differ in the 5′-untranslated region

David Crawford; Kathleen Hagerty; Bruce Beutler

doi:10.1016/0378-1119(89)90447-2

Multiple splice forms of ribonuclease-inhibitor mRNA differ in the 5′-untranslated region

David Crawford, Kathleen Hagerty, Bruce Beutler

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We report the sequence of the cDNAs representing five independent splice forms of human placental RNase inhibitor (RI) mRNA. RI mRNAs differ principally in the 5′-untranslated region, which may include or lack a 68-nucleotide (nt) exon inserted at a splice site located only 20 nt upstream from the initiator AUG. At least three other exons may also abut the same splice site. This unusual and variable feature of the mRNA would suggest that secondary structure in the region of the start codon may differ among RI messages. A single copy of the RI gene exists in the human genome.

Original language	English (US)
Pages (from-to)	525-531
Number of pages	7
Journal	Gene
Volume	85
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(89)90447-2
State	Published - Dec 28 1989

Keywords

RNase
Recombinant DNA
alterative splicing
cDNA
exon
human RNase inhibitor
start codon

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(89)90447-2

Cite this

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title = "Multiple splice forms of ribonuclease-inhibitor mRNA differ in the 5′-untranslated region",

abstract = "We report the sequence of the cDNAs representing five independent splice forms of human placental RNase inhibitor (RI) mRNA. RI mRNAs differ principally in the 5′-untranslated region, which may include or lack a 68-nucleotide (nt) exon inserted at a splice site located only 20 nt upstream from the initiator AUG. At least three other exons may also abut the same splice site. This unusual and variable feature of the mRNA would suggest that secondary structure in the region of the start codon may differ among RI messages. A single copy of the RI gene exists in the human genome.",

keywords = "RNase, Recombinant DNA, alterative splicing, cDNA, exon, human RNase inhibitor, start codon",

author = "David Crawford and Kathleen Hagerty and Bruce Beutler",

note = "Funding Information: This work was supported by NIH grant No. CA45525-03.W e are gratefult o Dr. Linette Caseyf or providingp lacentatl issuef or use in these studiesa nd to Ms. BetsyL ayton for her ables ecre-tarial assistance.",

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doi = "10.1016/0378-1119(89)90447-2",

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T1 - Multiple splice forms of ribonuclease-inhibitor mRNA differ in the 5′-untranslated region

AU - Crawford, David

AU - Hagerty, Kathleen

AU - Beutler, Bruce

N1 - Funding Information: This work was supported by NIH grant No. CA45525-03.W e are gratefult o Dr. Linette Caseyf or providingp lacentatl issuef or use in these studiesa nd to Ms. BetsyL ayton for her ables ecre-tarial assistance.

PY - 1989/12/28

Y1 - 1989/12/28

N2 - We report the sequence of the cDNAs representing five independent splice forms of human placental RNase inhibitor (RI) mRNA. RI mRNAs differ principally in the 5′-untranslated region, which may include or lack a 68-nucleotide (nt) exon inserted at a splice site located only 20 nt upstream from the initiator AUG. At least three other exons may also abut the same splice site. This unusual and variable feature of the mRNA would suggest that secondary structure in the region of the start codon may differ among RI messages. A single copy of the RI gene exists in the human genome.

AB - We report the sequence of the cDNAs representing five independent splice forms of human placental RNase inhibitor (RI) mRNA. RI mRNAs differ principally in the 5′-untranslated region, which may include or lack a 68-nucleotide (nt) exon inserted at a splice site located only 20 nt upstream from the initiator AUG. At least three other exons may also abut the same splice site. This unusual and variable feature of the mRNA would suggest that secondary structure in the region of the start codon may differ among RI messages. A single copy of the RI gene exists in the human genome.

KW - RNase

KW - Recombinant DNA

KW - alterative splicing

KW - cDNA

KW - exon

KW - human RNase inhibitor

KW - start codon

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AN - SCOPUS:0024845155

SN - 0378-1119

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EP - 531

JO - Gene

JF - Gene

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Multiple splice forms of ribonuclease-inhibitor mRNA differ in the 5′-untranslated region

Abstract

Keywords

ASJC Scopus subject areas

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